Cargando…

Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescen...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schulte, Roberta, Talamas, Jessica, Doucet, Christine, Hetzer, Martin W.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2008
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329591/ https://www.ncbi.nlm.nih.gov/pubmed/18446240 http://dx.doi.org/10.1371/journal.pone.0002061

Ejemplares similares

Sinbad, la leyenda de los siete mares = Sinbad, legend of the Seven Seas
Publicado: (2004)

SINBAD, structural, experimental and clinical characterization of STAT inhibitors and their potential applications
por: Plens-Gałąska, Martyna, et al.
Publicado: (2022)

Exploring functional variant discovery in non-coding regions with SInBaD
por: Lehmann, Kjong-Van, et al.
Publicado: (2013)

POM121 and Sun1 play a role in early steps of interphase NPC assembly
por: Talamas, Jessica A., et al.
Publicado: (2011)

A Bead Aggregation Assay for Detection of Low-Affinity Protein-Protein Interactions Reveals Interactions between N-Terminal Domains of Inositol 1,4,5-Trisphosphate Receptors
por: Chavda, Alap P., et al.
Publicado: (2013)

The Sinbad retrotransposon from the genome of the human blood fluke, Schistosoma mansoni, and the distribution of related Pao-like elements
por: Copeland, Claudia S, et al.
Publicado: (2005)

In vitro affinity screening of protein and peptide binders by megavalent bead surface display
por: Diamante, Letizia, et al.
Publicado: (2013)

Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads
por: Nguyen, Huy Quoc, et al.
Publicado: (2019)

FD5180, a Novel Protein Kinase Affinity Probe, and the Effect of Bead Loading on Protein Kinase Identification
por: Deane, Fiona M., et al.
Publicado: (2017)

Longitudinal phase space reconstruction simulation studies using a novel X-band transverse deflecting structure at the SINBAD facility at DESY
por: Marx, Daniel, et al.
Publicado: (2018)

Single-cell protein profiling in microchambers with barcoded beads
por: Armbrecht, Lucas, et al.
Publicado: (2019)

Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress
por: Buchwalter, Abigail, et al.
Publicado: (2019)

Analysis of Protein Interactions with Picomolar Binding Affinity by Fluorescence-Detected Sedimentation Velocity
por: Zhao, Huaying, et al.
Publicado: (2014)

Studying protein–protein affinity and immobilized ligand–protein affinity interactions using MS-based methods
por: Kool, Jeroen, et al.
Publicado: (2011)

Imaging Beads-Retained Prey Assay for Rapid and Quantitative Protein-Protein Interaction
por: Zhou, Yan, et al.
Publicado: (2013)

How Structure Defines Affinity in Protein-Protein Interactions
por: Erijman, Ariel, et al.
Publicado: (2014)

Interfacial Peptides as Affinity Modulating Agents of Protein-Protein Interactions
por: Ershov, Pavel V., et al.
Publicado: (2022)

A low-cost affinity purification system using β-1,3-glucan recognition protein and curdlan beads
por: Horiuchi, Masataka, et al.
Publicado: (2012)

Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice
por: Rohila, Jai S., et al.
Publicado: (2009)

Inflammatory and Angiogenic Protein Detection in the Human Vitreous: Cytometric Bead Assay
por: Koss, M. J., et al.
Publicado: (2011)

A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display
por: Sakihama, Toshiko, et al.
Publicado: (2008)

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays
por: Huovinen, Tuomas, et al.
Publicado: (2021)

High-Content Imaging for Large-Scale Detection of Low-Affinity Extracellular Protein Interactions
por: Wood, Laura, et al.
Publicado: (2019)

On the binding affinity of macromolecular interactions: daring to ask why proteins interact
por: Kastritis, Panagiotis L., et al.
Publicado: (2013)

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads
Publicado: (1984)

Characterization of Plasmodium Lactate Dehydrogenase and Histidine-Rich Protein 2 Clearance Patterns via Rapid On-Bead Detection from a Single Dried Blood Spot
por: Markwalter, Christine F., et al.
Publicado: (2018)

Performance of dye-affinity beads for aluminium removal in magnetically stabilized fluidized bed
por: Yavuz, Handan, et al.
Publicado: (2004)

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification
por: Liao, Pin-Chao, et al.
Publicado: (2018)

Microchip capillary gel electrophoresis combined with lectin affinity enrichment employing magnetic beads for glycoprotein analysis
por: Engel, Nicole Y., et al.
Publicado: (2017)

Targeting Protein-Protein Interactions with Trimeric Ligands: High Affinity Inhibitors of the MAGUK Protein Family
por: Nissen, Klaus B., et al.
Publicado: (2015)

Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes
por: Trinkle-Mulcahy, Laura, et al.
Publicado: (2008)

Formation of stacked ER cisternae by low affinity protein interactions
por: Snapp, Erik L., et al.
Publicado: (2003)

Identification of Protein Interacting Partners Using Tandem Affinity Purification
por: Bailey, Dalan, et al.
Publicado: (2012)

Approaching infinite affinity through engineering of peptide–protein interaction
por: Keeble, Anthony H., et al.
Publicado: (2019)

Leveraging Affinity Interactions to Prolong Drug Delivery of Protein Therapeutics
por: Dogan, Alan B., et al.
Publicado: (2022)

Development of a multiplexed bead-based immunoassay for the simultaneous detection of antibodies to 17 pneumococcal proteins
por: Shoma, S., et al.
Publicado: (2010)

Nuclear pore proteins and the control of genome functions
por: Ibarra, Arkaitz, et al.
Publicado: (2015)

Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography
Publicado: (1989)

Magneto-nanosensor platform for probing low-affinity protein–protein interactions and identification of a low-affinity PD-L1/PD-L2 interaction
por: Lee, Jung-Rok, et al.
Publicado: (2016)

Identifying binary protein-protein interactions from affinity purification mass spectrometry data
por: Zhang, Xiao-Fei, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_7ublrc13n234pqi6ggie5o37t2