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The 3′ processing factor CstF functions in the DNA repair response
Following DNA damage, mRNA levels decrease, reflecting a coordinated interaction of the DNA repair, transcription and RNA processing machineries. In this study, we provide evidence that transcription and polyadenylation of mRNA precursors are both affected in vivo by UV treatment. We next show that...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330234/ https://www.ncbi.nlm.nih.gov/pubmed/18252771 http://dx.doi.org/10.1093/nar/gkn005 |
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author | Mirkin, Nurit Fonseca, Danae Mohammed, Samia Cevher, Murat A. Manley, James L. Kleiman, Frida E. |
author_facet | Mirkin, Nurit Fonseca, Danae Mohammed, Samia Cevher, Murat A. Manley, James L. Kleiman, Frida E. |
author_sort | Mirkin, Nurit |
collection | PubMed |
description | Following DNA damage, mRNA levels decrease, reflecting a coordinated interaction of the DNA repair, transcription and RNA processing machineries. In this study, we provide evidence that transcription and polyadenylation of mRNA precursors are both affected in vivo by UV treatment. We next show that the polyadenylation factor CstF, plays a direct role in the DNA damage response. Cells with reduced levels of CstF display decreased viability following UV treatment, reduced ability to ubiquitinate RNA polymerase II (RNAP II), and defects in repair of DNA damage. Furthermore, we show that CstF, RNAP II and BARD1 are all found at sites of repaired DNA. Our results indicate that CstF plays an active role in the response to DNA damage, providing a link between transcription-coupled RNA processing and DNA repair. |
format | Text |
id | pubmed-2330234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-23302342008-05-05 The 3′ processing factor CstF functions in the DNA repair response Mirkin, Nurit Fonseca, Danae Mohammed, Samia Cevher, Murat A. Manley, James L. Kleiman, Frida E. Nucleic Acids Res RNA Following DNA damage, mRNA levels decrease, reflecting a coordinated interaction of the DNA repair, transcription and RNA processing machineries. In this study, we provide evidence that transcription and polyadenylation of mRNA precursors are both affected in vivo by UV treatment. We next show that the polyadenylation factor CstF, plays a direct role in the DNA damage response. Cells with reduced levels of CstF display decreased viability following UV treatment, reduced ability to ubiquitinate RNA polymerase II (RNAP II), and defects in repair of DNA damage. Furthermore, we show that CstF, RNAP II and BARD1 are all found at sites of repaired DNA. Our results indicate that CstF plays an active role in the response to DNA damage, providing a link between transcription-coupled RNA processing and DNA repair. Oxford University Press 2008-04 2008-02-05 /pmc/articles/PMC2330234/ /pubmed/18252771 http://dx.doi.org/10.1093/nar/gkn005 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Mirkin, Nurit Fonseca, Danae Mohammed, Samia Cevher, Murat A. Manley, James L. Kleiman, Frida E. The 3′ processing factor CstF functions in the DNA repair response |
title | The 3′ processing factor CstF functions in the DNA repair response |
title_full | The 3′ processing factor CstF functions in the DNA repair response |
title_fullStr | The 3′ processing factor CstF functions in the DNA repair response |
title_full_unstemmed | The 3′ processing factor CstF functions in the DNA repair response |
title_short | The 3′ processing factor CstF functions in the DNA repair response |
title_sort | 3′ processing factor cstf functions in the dna repair response |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330234/ https://www.ncbi.nlm.nih.gov/pubmed/18252771 http://dx.doi.org/10.1093/nar/gkn005 |
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