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Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection
BACKGROUND: Transcriptomic approaches are relevant for studying virus-host cell dialogues to better understand the physiopathology of infection and the immune response at the cellular level. Pseudorabies virus (PrV), a porcine Alphaherpesvirus, is a good model for such studies in pig. Since PrV disp...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335119/ https://www.ncbi.nlm.nih.gov/pubmed/18331636 http://dx.doi.org/10.1186/1471-2164-9-123 |
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author | Flori, Laurence Rogel-Gaillard, Claire Cochet, Marielle Lemonnier, Gaetan Hugot, Karine Chardon, Patrick Robin, Stéphane Lefèvre, François |
author_facet | Flori, Laurence Rogel-Gaillard, Claire Cochet, Marielle Lemonnier, Gaetan Hugot, Karine Chardon, Patrick Robin, Stéphane Lefèvre, François |
author_sort | Flori, Laurence |
collection | PubMed |
description | BACKGROUND: Transcriptomic approaches are relevant for studying virus-host cell dialogues to better understand the physiopathology of infection and the immune response at the cellular level. Pseudorabies virus (PrV), a porcine Alphaherpesvirus, is a good model for such studies in pig. Since PrV displays a strong tropism for mucous epithelial cells, we developed a kinetics study of PrV infection in the porcine PK15 epithelial cell line. To identify as completely as possible, viral and cellular genes regulated during infection, we simultaneously analyzed PrV and cellular transcriptome modifications using two microarrays i.e. a laboratory-made combined SLA/PrV microarray, consisting of probes for all PrV genes and for porcine genes contained in the Swine Leukocyte Antigen (SLA) complex, and the porcine generic Qiagen-NRSP8 oligonucleotide microarray. We confirmed the differential expression of a selected set of genes by qRT-PCR and flow cytometry. RESULTS: An increase in the number of differentially expressed cellular genes and PrV genes especially from 4 h post-infection (pi) was observed concomitantly with the onset of viral progeny while no early global cellular shutoff was recorded. Many cellular genes were down-regulated from 4 h pi and their number increased until 12 h pi. UL41 transcripts encoding the virion host shutoff protein were first detected as differentially expressed at 8 h pi. The viral gene UL49.5 encoding a TAP inhibitor protein was differentially expressed as soon as 2 h pi, indicating that viral evasion via TAP inhibition may start earlier than the cellular gene shutoff. We found that many biological processes are altered during PrV infection. Indeed, several genes involved in the SLA class I antigenic presentation pathway (SLA-Ia, TAP1, TAP2, PSMB8 and PSMB9), were down-regulated, thus contributing to viral immune escape from this pathway and other genes involved in apoptosis, nucleic acid metabolism, cytoskeleton signaling as well as interferon-mediated antiviral response were also modulated during PrV infection. CONCLUSION: Our results show that the gene expression of both PrV and porcine cells can be analyzed simultaneously with microarrays, providing a chronology of PrV gene transcription, which has never been described before, and a global picture of transcription with a direct temporal link between viral and host gene expression. |
format | Text |
id | pubmed-2335119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-23351192008-04-25 Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection Flori, Laurence Rogel-Gaillard, Claire Cochet, Marielle Lemonnier, Gaetan Hugot, Karine Chardon, Patrick Robin, Stéphane Lefèvre, François BMC Genomics Research Article BACKGROUND: Transcriptomic approaches are relevant for studying virus-host cell dialogues to better understand the physiopathology of infection and the immune response at the cellular level. Pseudorabies virus (PrV), a porcine Alphaherpesvirus, is a good model for such studies in pig. Since PrV displays a strong tropism for mucous epithelial cells, we developed a kinetics study of PrV infection in the porcine PK15 epithelial cell line. To identify as completely as possible, viral and cellular genes regulated during infection, we simultaneously analyzed PrV and cellular transcriptome modifications using two microarrays i.e. a laboratory-made combined SLA/PrV microarray, consisting of probes for all PrV genes and for porcine genes contained in the Swine Leukocyte Antigen (SLA) complex, and the porcine generic Qiagen-NRSP8 oligonucleotide microarray. We confirmed the differential expression of a selected set of genes by qRT-PCR and flow cytometry. RESULTS: An increase in the number of differentially expressed cellular genes and PrV genes especially from 4 h post-infection (pi) was observed concomitantly with the onset of viral progeny while no early global cellular shutoff was recorded. Many cellular genes were down-regulated from 4 h pi and their number increased until 12 h pi. UL41 transcripts encoding the virion host shutoff protein were first detected as differentially expressed at 8 h pi. The viral gene UL49.5 encoding a TAP inhibitor protein was differentially expressed as soon as 2 h pi, indicating that viral evasion via TAP inhibition may start earlier than the cellular gene shutoff. We found that many biological processes are altered during PrV infection. Indeed, several genes involved in the SLA class I antigenic presentation pathway (SLA-Ia, TAP1, TAP2, PSMB8 and PSMB9), were down-regulated, thus contributing to viral immune escape from this pathway and other genes involved in apoptosis, nucleic acid metabolism, cytoskeleton signaling as well as interferon-mediated antiviral response were also modulated during PrV infection. CONCLUSION: Our results show that the gene expression of both PrV and porcine cells can be analyzed simultaneously with microarrays, providing a chronology of PrV gene transcription, which has never been described before, and a global picture of transcription with a direct temporal link between viral and host gene expression. BioMed Central 2008-03-10 /pmc/articles/PMC2335119/ /pubmed/18331636 http://dx.doi.org/10.1186/1471-2164-9-123 Text en Copyright © 2008 Flori et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Flori, Laurence Rogel-Gaillard, Claire Cochet, Marielle Lemonnier, Gaetan Hugot, Karine Chardon, Patrick Robin, Stéphane Lefèvre, François Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title | Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title_full | Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title_fullStr | Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title_full_unstemmed | Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title_short | Transcriptomic analysis of the dialogue between Pseudorabies virus and porcine epithelial cells during infection |
title_sort | transcriptomic analysis of the dialogue between pseudorabies virus and porcine epithelial cells during infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335119/ https://www.ncbi.nlm.nih.gov/pubmed/18331636 http://dx.doi.org/10.1186/1471-2164-9-123 |
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