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SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2
BACKGROUND: Streptococcus suis serotype 2 (S. suis 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by a toxic shock-like syndrome. However, the molecular pathogenesis of this new emerging path...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2358977/ https://www.ncbi.nlm.nih.gov/pubmed/18461172 http://dx.doi.org/10.1371/journal.pone.0002080 |
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author | Li, Ming Wang, Changjun Feng, Youjun Pan, Xiuzhen Cheng, Gong Wang, Jing Ge, Junchao Zheng, Feng Cao, Min Dong, Yaqing Liu, Di Wang, Jufang Lin, Ying Du, Hongli Gao, George F. Wang, Xiaoning Hu, Fuquan Tang, Jiaqi |
author_facet | Li, Ming Wang, Changjun Feng, Youjun Pan, Xiuzhen Cheng, Gong Wang, Jing Ge, Junchao Zheng, Feng Cao, Min Dong, Yaqing Liu, Di Wang, Jufang Lin, Ying Du, Hongli Gao, George F. Wang, Xiaoning Hu, Fuquan Tang, Jiaqi |
author_sort | Li, Ming |
collection | PubMed |
description | BACKGROUND: Streptococcus suis serotype 2 (S. suis 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by a toxic shock-like syndrome. However, the molecular pathogenesis of this new emerging pathogen is still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: 89K is a newly predicted pathogenicity island (PAI) which is specific to Chinese epidemic strains isolated from these two SS2 outbreaks. Further bioinformatics analysis revealed a unique two-component signal transduction system (TCSTS) located in the candidate 89K PAI, which is orthologous to the SalK/SalR regulatory system of Streptococcus salivarius. Knockout of salKR eliminated the lethality of SS2 in experimental infection of piglets. Functional complementation of salKR into the isogenic mutant ΔsalKR restored its soaring pathogenicity. Colonization experiments showed that the ΔsalKR mutant could not colonize any susceptible tissue of piglets when administered alone. Bactericidal assays demonstrated that resistance of the mutant to polymorphonuclear leukocyte (PMN)-mediated killing was greatly decreased. Expression microarray analysis exhibited a transcription profile alteration of 26 various genes down-regulated in the ΔsalKR mutant. CONCLUSIONS/SIGNIFICANCE: These findings suggest that SalK/SalR is requisite for the full virulence of ethnic Chinese isolates of highly pathogenic SS2, thus providing experimental evidence for the validity of this bioinformatically predicted PAI. |
format | Text |
id | pubmed-2358977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-23589772008-05-07 SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 Li, Ming Wang, Changjun Feng, Youjun Pan, Xiuzhen Cheng, Gong Wang, Jing Ge, Junchao Zheng, Feng Cao, Min Dong, Yaqing Liu, Di Wang, Jufang Lin, Ying Du, Hongli Gao, George F. Wang, Xiaoning Hu, Fuquan Tang, Jiaqi PLoS One Research Article BACKGROUND: Streptococcus suis serotype 2 (S. suis 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by a toxic shock-like syndrome. However, the molecular pathogenesis of this new emerging pathogen is still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: 89K is a newly predicted pathogenicity island (PAI) which is specific to Chinese epidemic strains isolated from these two SS2 outbreaks. Further bioinformatics analysis revealed a unique two-component signal transduction system (TCSTS) located in the candidate 89K PAI, which is orthologous to the SalK/SalR regulatory system of Streptococcus salivarius. Knockout of salKR eliminated the lethality of SS2 in experimental infection of piglets. Functional complementation of salKR into the isogenic mutant ΔsalKR restored its soaring pathogenicity. Colonization experiments showed that the ΔsalKR mutant could not colonize any susceptible tissue of piglets when administered alone. Bactericidal assays demonstrated that resistance of the mutant to polymorphonuclear leukocyte (PMN)-mediated killing was greatly decreased. Expression microarray analysis exhibited a transcription profile alteration of 26 various genes down-regulated in the ΔsalKR mutant. CONCLUSIONS/SIGNIFICANCE: These findings suggest that SalK/SalR is requisite for the full virulence of ethnic Chinese isolates of highly pathogenic SS2, thus providing experimental evidence for the validity of this bioinformatically predicted PAI. Public Library of Science 2008-05-07 /pmc/articles/PMC2358977/ /pubmed/18461172 http://dx.doi.org/10.1371/journal.pone.0002080 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Ming Wang, Changjun Feng, Youjun Pan, Xiuzhen Cheng, Gong Wang, Jing Ge, Junchao Zheng, Feng Cao, Min Dong, Yaqing Liu, Di Wang, Jufang Lin, Ying Du, Hongli Gao, George F. Wang, Xiaoning Hu, Fuquan Tang, Jiaqi SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title | SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title_full | SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title_fullStr | SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title_full_unstemmed | SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title_short | SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2 |
title_sort | salk/salr, a two-component signal transduction system, is essential for full virulence of highly invasive streptococcus suis serotype 2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2358977/ https://www.ncbi.nlm.nih.gov/pubmed/18461172 http://dx.doi.org/10.1371/journal.pone.0002080 |
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