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Genomic copy number and expression patterns in testicular germ cell tumours
Testicular germ cell tumours of adults and adolescents (TGCT) include seminomas (SE) and nonseminomas (NS), with spermatocytic seminomas (SSE) representing a distinct entity in older men. SE and NS have gain of 12p material in all cases, whereas SSE are associated with overrepresentation of chromoso...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360290/ https://www.ncbi.nlm.nih.gov/pubmed/18059402 http://dx.doi.org/10.1038/sj.bjc.6604079 |
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author | McIntyre, A Summersgill, B Lu, Y J Missiaglia, E Kitazawa, S Oosterhuis, J W Looijenga, L H Shipley, J |
author_facet | McIntyre, A Summersgill, B Lu, Y J Missiaglia, E Kitazawa, S Oosterhuis, J W Looijenga, L H Shipley, J |
author_sort | McIntyre, A |
collection | PubMed |
description | Testicular germ cell tumours of adults and adolescents (TGCT) include seminomas (SE) and nonseminomas (NS), with spermatocytic seminomas (SSE) representing a distinct entity in older men. SE and NS have gain of 12p material in all cases, whereas SSE are associated with overrepresentation of chromosome 9. Here, we compare at the chromosomal level, copy number imbalances with global expression changes, identified by comparative expressed sequence hybridisation analyses, in seven SE, one combined tumour, seven NS and seven cell lines. Positive correlations were found consistent with copy number as a main driver of expression change, despite reported differences in methylation status in SE and NS. Analysis of chromosomal copy number and expression data could not distinguish between SE and NS, in-keeping with a similar genetic pathogenesis. However, increased expression from 4q22, 5q23.2 and 9p21 distinguished SSE from SE and NS and decreased copy number and expression from 2q36–q37 and 6q24 was a specific feature of NS-derived cell lines. Our analysis also highlights 19 regions with both copy number and expression imbalances in greater than 40% of cases. Mining available expression array data identified genes from these regions as candidates for involvement in TGCT development. Supplementary data is available at http://www.crukdmf.icr.ac.uk/array/array.html. |
format | Text |
id | pubmed-2360290 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23602902009-09-10 Genomic copy number and expression patterns in testicular germ cell tumours McIntyre, A Summersgill, B Lu, Y J Missiaglia, E Kitazawa, S Oosterhuis, J W Looijenga, L H Shipley, J Br J Cancer Molecular Diagnostics Testicular germ cell tumours of adults and adolescents (TGCT) include seminomas (SE) and nonseminomas (NS), with spermatocytic seminomas (SSE) representing a distinct entity in older men. SE and NS have gain of 12p material in all cases, whereas SSE are associated with overrepresentation of chromosome 9. Here, we compare at the chromosomal level, copy number imbalances with global expression changes, identified by comparative expressed sequence hybridisation analyses, in seven SE, one combined tumour, seven NS and seven cell lines. Positive correlations were found consistent with copy number as a main driver of expression change, despite reported differences in methylation status in SE and NS. Analysis of chromosomal copy number and expression data could not distinguish between SE and NS, in-keeping with a similar genetic pathogenesis. However, increased expression from 4q22, 5q23.2 and 9p21 distinguished SSE from SE and NS and decreased copy number and expression from 2q36–q37 and 6q24 was a specific feature of NS-derived cell lines. Our analysis also highlights 19 regions with both copy number and expression imbalances in greater than 40% of cases. Mining available expression array data identified genes from these regions as candidates for involvement in TGCT development. Supplementary data is available at http://www.crukdmf.icr.ac.uk/array/array.html. Nature Publishing Group 2007-12-17 2007-12-04 /pmc/articles/PMC2360290/ /pubmed/18059402 http://dx.doi.org/10.1038/sj.bjc.6604079 Text en Copyright © 2007 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics McIntyre, A Summersgill, B Lu, Y J Missiaglia, E Kitazawa, S Oosterhuis, J W Looijenga, L H Shipley, J Genomic copy number and expression patterns in testicular germ cell tumours |
title | Genomic copy number and expression patterns in testicular germ cell tumours |
title_full | Genomic copy number and expression patterns in testicular germ cell tumours |
title_fullStr | Genomic copy number and expression patterns in testicular germ cell tumours |
title_full_unstemmed | Genomic copy number and expression patterns in testicular germ cell tumours |
title_short | Genomic copy number and expression patterns in testicular germ cell tumours |
title_sort | genomic copy number and expression patterns in testicular germ cell tumours |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360290/ https://www.ncbi.nlm.nih.gov/pubmed/18059402 http://dx.doi.org/10.1038/sj.bjc.6604079 |
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