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Increased Dickkopf-1 expression in breast cancer bone metastases

The aim of this study was to determine whether Dickkopf-1 (Dkk-1) expression in breast cancer was associated with bone metastases. We first analysed Dkk-1 expression by human breast cancer cell lines that induce osteolytic or osteoblastic lesions in animals. Dickkopf-1 levels were then measured in t...

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Autores principales: Voorzanger-Rousselot, N, Goehrig, D, Journe, F, Doriath, V, Body, J J, Clézardin, P, Garnero, P
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2007
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360424/
https://www.ncbi.nlm.nih.gov/pubmed/17876334
http://dx.doi.org/10.1038/sj.bjc.6603959
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author Voorzanger-Rousselot, N
Goehrig, D
Journe, F
Doriath, V
Body, J J
Clézardin, P
Garnero, P
author_facet Voorzanger-Rousselot, N
Goehrig, D
Journe, F
Doriath, V
Body, J J
Clézardin, P
Garnero, P
author_sort Voorzanger-Rousselot, N
collection PubMed
description The aim of this study was to determine whether Dickkopf-1 (Dkk-1) expression in breast cancer was associated with bone metastases. We first analysed Dkk-1 expression by human breast cancer cell lines that induce osteolytic or osteoblastic lesions in animals. Dickkopf-1 levels were then measured in the bone marrow aspirates of hind limbs from eight NMRI mice inoculated with breast cancer cells that induced bone metastases and 11 age-matched non-inoculated control animals. Finally, Dkk-1 was measured in the serum of 17 women with breast cancer in complete remission, 19 women with breast cancer and bone metastases, 16 women with breast cancer and metastases at non-bone sites and 16 healthy women. Only breast cancer cells that induce osteolytic lesions in animals produced Dkk-1. There was a six-fold increase in Dkk-1 levels in the bone marrow from animals inoculated with MDA-B02 cells when compared with that of control non-inoculated animals (P=0.003). Median Dkk-1 levels in the serum of patients with breast cancer and bone metastases were significantly higher than levels of patients in complete remission (P=0.016), patients with breast cancer having metastases at non-bone sites (P<0.0001) and healthy women (P=0.047), although there was a large overlap in individual levels between the different groups. In conclusion, Dkk-1 is secreted by osteolytic human breast cancer cells lines and increased circulating levels are associated with the presence of bone metastases in patients with breast cancer. Measurements of circulating Dkk-1 levels may be useful for the clinical investigation of patients with breast cancer and bone metastases.
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spelling pubmed-23604242009-09-10 Increased Dickkopf-1 expression in breast cancer bone metastases Voorzanger-Rousselot, N Goehrig, D Journe, F Doriath, V Body, J J Clézardin, P Garnero, P Br J Cancer Molecular Diagnostics The aim of this study was to determine whether Dickkopf-1 (Dkk-1) expression in breast cancer was associated with bone metastases. We first analysed Dkk-1 expression by human breast cancer cell lines that induce osteolytic or osteoblastic lesions in animals. Dickkopf-1 levels were then measured in the bone marrow aspirates of hind limbs from eight NMRI mice inoculated with breast cancer cells that induced bone metastases and 11 age-matched non-inoculated control animals. Finally, Dkk-1 was measured in the serum of 17 women with breast cancer in complete remission, 19 women with breast cancer and bone metastases, 16 women with breast cancer and metastases at non-bone sites and 16 healthy women. Only breast cancer cells that induce osteolytic lesions in animals produced Dkk-1. There was a six-fold increase in Dkk-1 levels in the bone marrow from animals inoculated with MDA-B02 cells when compared with that of control non-inoculated animals (P=0.003). Median Dkk-1 levels in the serum of patients with breast cancer and bone metastases were significantly higher than levels of patients in complete remission (P=0.016), patients with breast cancer having metastases at non-bone sites (P<0.0001) and healthy women (P=0.047), although there was a large overlap in individual levels between the different groups. In conclusion, Dkk-1 is secreted by osteolytic human breast cancer cells lines and increased circulating levels are associated with the presence of bone metastases in patients with breast cancer. Measurements of circulating Dkk-1 levels may be useful for the clinical investigation of patients with breast cancer and bone metastases. Nature Publishing Group 2007-10-08 2007-09-18 /pmc/articles/PMC2360424/ /pubmed/17876334 http://dx.doi.org/10.1038/sj.bjc.6603959 Text en Copyright © 2007 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Voorzanger-Rousselot, N
Goehrig, D
Journe, F
Doriath, V
Body, J J
Clézardin, P
Garnero, P
Increased Dickkopf-1 expression in breast cancer bone metastases
title Increased Dickkopf-1 expression in breast cancer bone metastases
title_full Increased Dickkopf-1 expression in breast cancer bone metastases
title_fullStr Increased Dickkopf-1 expression in breast cancer bone metastases
title_full_unstemmed Increased Dickkopf-1 expression in breast cancer bone metastases
title_short Increased Dickkopf-1 expression in breast cancer bone metastases
title_sort increased dickkopf-1 expression in breast cancer bone metastases
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360424/
https://www.ncbi.nlm.nih.gov/pubmed/17876334
http://dx.doi.org/10.1038/sj.bjc.6603959
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