Cargando…
C4.4A as a candidate marker in the diagnosis of colorectal cancer
C4.4A is a member of the Ly-6 family with restricted expression in non-transformed tissues. C4.4A expression in human cancer has rarely been evaluated. Thus, it became important to explore C4.4A protein expression in human tumour tissue to obtain an estimate on the frequency of expression and the co...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360445/ https://www.ncbi.nlm.nih.gov/pubmed/17912244 http://dx.doi.org/10.1038/sj.bjc.6604012 |
_version_ | 1782153051275001856 |
---|---|
author | Paret, C Hildebrand, D Weitz, J Kopp-Schneider, A Kuhn, A Beer, A Hautmann, R Zöller, M |
author_facet | Paret, C Hildebrand, D Weitz, J Kopp-Schneider, A Kuhn, A Beer, A Hautmann, R Zöller, M |
author_sort | Paret, C |
collection | PubMed |
description | C4.4A is a member of the Ly-6 family with restricted expression in non-transformed tissues. C4.4A expression in human cancer has rarely been evaluated. Thus, it became important to explore C4.4A protein expression in human tumour tissue to obtain an estimate on the frequency of expression and the correlation with tumour progression, the study focusing on colorectal cancer. The analysis of C4.4A in human tumour lines by western blot and immunoprecipitation using polyclonal rabbit antibodies that recognize different C4.4A epitopes revealed C4.4A oligomer and heavily glycosylated C4.4A isoform expression that, in some instances, inhibited antibody binding and interaction with the C4.4A ligand galectin-3. In addition, tumour cell lines released C4.4A by vesicle shedding and proteolytic cleavage. C4.4A was expressed in over 80% of primary colorectal cancer and liver metastasis with negligible expression in adjacent colonic mucosa, inflamed colonic tissue and liver. This compares well with EpCAM and CO-029 expression in over 90% of colorectal cancer. C4.4A expression was only observed in about 50% of pancreatic cancer and renal cell carcinoma. By de novo expression in colonic cancer tissue, we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids. |
format | Text |
id | pubmed-2360445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23604452009-09-10 C4.4A as a candidate marker in the diagnosis of colorectal cancer Paret, C Hildebrand, D Weitz, J Kopp-Schneider, A Kuhn, A Beer, A Hautmann, R Zöller, M Br J Cancer Molecular Diagnostics C4.4A is a member of the Ly-6 family with restricted expression in non-transformed tissues. C4.4A expression in human cancer has rarely been evaluated. Thus, it became important to explore C4.4A protein expression in human tumour tissue to obtain an estimate on the frequency of expression and the correlation with tumour progression, the study focusing on colorectal cancer. The analysis of C4.4A in human tumour lines by western blot and immunoprecipitation using polyclonal rabbit antibodies that recognize different C4.4A epitopes revealed C4.4A oligomer and heavily glycosylated C4.4A isoform expression that, in some instances, inhibited antibody binding and interaction with the C4.4A ligand galectin-3. In addition, tumour cell lines released C4.4A by vesicle shedding and proteolytic cleavage. C4.4A was expressed in over 80% of primary colorectal cancer and liver metastasis with negligible expression in adjacent colonic mucosa, inflamed colonic tissue and liver. This compares well with EpCAM and CO-029 expression in over 90% of colorectal cancer. C4.4A expression was only observed in about 50% of pancreatic cancer and renal cell carcinoma. By de novo expression in colonic cancer tissue, we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids. Nature Publishing Group 2007-10-22 2007-10-02 /pmc/articles/PMC2360445/ /pubmed/17912244 http://dx.doi.org/10.1038/sj.bjc.6604012 Text en Copyright © 2007 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Paret, C Hildebrand, D Weitz, J Kopp-Schneider, A Kuhn, A Beer, A Hautmann, R Zöller, M C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title | C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title_full | C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title_fullStr | C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title_full_unstemmed | C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title_short | C4.4A as a candidate marker in the diagnosis of colorectal cancer |
title_sort | c4.4a as a candidate marker in the diagnosis of colorectal cancer |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360445/ https://www.ncbi.nlm.nih.gov/pubmed/17912244 http://dx.doi.org/10.1038/sj.bjc.6604012 |
work_keys_str_mv | AT paretc c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT hildebrandd c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT weitzj c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT koppschneidera c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT kuhna c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT beera c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT hautmannr c44aasacandidatemarkerinthediagnosisofcolorectalcancer AT zollerm c44aasacandidatemarkerinthediagnosisofcolorectalcancer |