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Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status

Human kallikrein 14 (KLK14) is a steroid hormone-regulated member of the tissue kallikrein family of serine proteases, for which a prognostic and diagnostic value in breast cancer has been suggested. To further characterise the value of KLK14 as a breast tumour marker, we have carefully analysed KLK...

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Autores principales: Fritzsche, F, Gansukh, T, Borgoño, C A, Burkhardt, M, Pahl, S, Mayordomo, E, Winzer, K-J, Weichert, W, Denkert, C, Jung, K, Stephan, C, Dietel, M, Diamandis, E P, Dahl, E, Kristiansen, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361186/
https://www.ncbi.nlm.nih.gov/pubmed/16434994
http://dx.doi.org/10.1038/sj.bjc.6602956
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author Fritzsche, F
Gansukh, T
Borgoño, C A
Burkhardt, M
Pahl, S
Mayordomo, E
Winzer, K-J
Weichert, W
Denkert, C
Jung, K
Stephan, C
Dietel, M
Diamandis, E P
Dahl, E
Kristiansen, G
author_facet Fritzsche, F
Gansukh, T
Borgoño, C A
Burkhardt, M
Pahl, S
Mayordomo, E
Winzer, K-J
Weichert, W
Denkert, C
Jung, K
Stephan, C
Dietel, M
Diamandis, E P
Dahl, E
Kristiansen, G
author_sort Fritzsche, F
collection PubMed
description Human kallikrein 14 (KLK14) is a steroid hormone-regulated member of the tissue kallikrein family of serine proteases, for which a prognostic and diagnostic value in breast cancer has been suggested. To further characterise the value of KLK14 as a breast tumour marker, we have carefully analysed KLK14 expression in normal breast tissue and breast cancer both on the RNA level by real-time RT-PCR (n=39), and on the protein level (n=127) using a KLK14-specific antibody for immunohistochemistry. We correlated KLK14 protein expression data with available clinico-pathological parameters (mean follow-up time was 55 months) including patient prognosis. KLK14 RNA expression as quantified by real-time RT-PCR was significantly more abundant in breast tumours compared to normal breast tissue (P=0.027), an issue that had not been clarified recently. Concordantly with the RNA data, cytoplasmic KLK14 protein expression was significantly higher in invasive breast carcinomas compared to normal breast tissues (P=0.003). Furthermore, KLK14 protein expression was associated with higher tumour grade (P=0.041) and positive nodal status (P=0.045) but was not significantly associated with shortened disease-free or overall patient survival time in univariate analyses. We conclude that KLK14 is clearly overexpressed in breast cancer in comparison to normal breast tissues and is positively associated with conventional parameters of tumour aggressiveness, but due to a missing association with survival times, the use of KLK14 immunohistochemistry as a prognostic marker in breast cancer is questionable.
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spelling pubmed-23611862009-09-10 Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status Fritzsche, F Gansukh, T Borgoño, C A Burkhardt, M Pahl, S Mayordomo, E Winzer, K-J Weichert, W Denkert, C Jung, K Stephan, C Dietel, M Diamandis, E P Dahl, E Kristiansen, G Br J Cancer Molecular Diagnostics Human kallikrein 14 (KLK14) is a steroid hormone-regulated member of the tissue kallikrein family of serine proteases, for which a prognostic and diagnostic value in breast cancer has been suggested. To further characterise the value of KLK14 as a breast tumour marker, we have carefully analysed KLK14 expression in normal breast tissue and breast cancer both on the RNA level by real-time RT-PCR (n=39), and on the protein level (n=127) using a KLK14-specific antibody for immunohistochemistry. We correlated KLK14 protein expression data with available clinico-pathological parameters (mean follow-up time was 55 months) including patient prognosis. KLK14 RNA expression as quantified by real-time RT-PCR was significantly more abundant in breast tumours compared to normal breast tissue (P=0.027), an issue that had not been clarified recently. Concordantly with the RNA data, cytoplasmic KLK14 protein expression was significantly higher in invasive breast carcinomas compared to normal breast tissues (P=0.003). Furthermore, KLK14 protein expression was associated with higher tumour grade (P=0.041) and positive nodal status (P=0.045) but was not significantly associated with shortened disease-free or overall patient survival time in univariate analyses. We conclude that KLK14 is clearly overexpressed in breast cancer in comparison to normal breast tissues and is positively associated with conventional parameters of tumour aggressiveness, but due to a missing association with survival times, the use of KLK14 immunohistochemistry as a prognostic marker in breast cancer is questionable. Nature Publishing Group 2006-02-27 2006-01-24 /pmc/articles/PMC2361186/ /pubmed/16434994 http://dx.doi.org/10.1038/sj.bjc.6602956 Text en Copyright © 2006 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Fritzsche, F
Gansukh, T
Borgoño, C A
Burkhardt, M
Pahl, S
Mayordomo, E
Winzer, K-J
Weichert, W
Denkert, C
Jung, K
Stephan, C
Dietel, M
Diamandis, E P
Dahl, E
Kristiansen, G
Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title_full Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title_fullStr Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title_full_unstemmed Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title_short Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status
title_sort expression of human kallikrein 14 (klk14) in breast cancer is associated with higher tumour grades and positive nodal status
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361186/
https://www.ncbi.nlm.nih.gov/pubmed/16434994
http://dx.doi.org/10.1038/sj.bjc.6602956
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