Cargando…
Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein is an RNA-binding protein involved in many processes that compose gene expression. K protein is upregulated in the malignant processes and has been shown to modulate the expression of genes involved in mitogenic responses and tumorigene...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2006
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361188/ https://www.ncbi.nlm.nih.gov/pubmed/16404425 http://dx.doi.org/10.1038/sj.bjc.6602938 |
_version_ | 1782153154955051008 |
---|---|
author | Klimek-Tomczak, K Mikula, M Dzwonek, A Paziewska, A Karczmarski, J Hennig, E Bujnicki, J M Brągoszewski, P Denisenko, O Bomsztyk, K Ostrowski, J |
author_facet | Klimek-Tomczak, K Mikula, M Dzwonek, A Paziewska, A Karczmarski, J Hennig, E Bujnicki, J M Brągoszewski, P Denisenko, O Bomsztyk, K Ostrowski, J |
author_sort | Klimek-Tomczak, K |
collection | PubMed |
description | The heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein is an RNA-binding protein involved in many processes that compose gene expression. K protein is upregulated in the malignant processes and has been shown to modulate the expression of genes involved in mitogenic responses and tumorigenesis. To explore the possibility that there are alternative isoforms of K protein expressed in colon cancer, we amplified and sequenced K protein mRNA that was isolated from colorectal cancers as well as from normal tissues surrounding the tumours. Sequencing revealed a single G-to-A base substitution at position 274 that was found in tumours and surrounding mucosa, but not in individuals that had no colorectal tumour. This substitution most likely reflects an RNA editing event because it was not found in the corresponding genomic DNAs. Sequencing of RNA from normal colonic mucosa of patients with prior resection of colorectal cancer revealed only the wild-type K protein transcript, indicating that G274A isoform is tumour related. To our knowledge, this is the first example of an RNA editing event in cancer and its surrounding tissue, a finding that may offer a new diagnostic and treatment marker. |
format | Text |
id | pubmed-2361188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23611882009-09-10 Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa Klimek-Tomczak, K Mikula, M Dzwonek, A Paziewska, A Karczmarski, J Hennig, E Bujnicki, J M Brągoszewski, P Denisenko, O Bomsztyk, K Ostrowski, J Br J Cancer Genetics and Genomics The heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein is an RNA-binding protein involved in many processes that compose gene expression. K protein is upregulated in the malignant processes and has been shown to modulate the expression of genes involved in mitogenic responses and tumorigenesis. To explore the possibility that there are alternative isoforms of K protein expressed in colon cancer, we amplified and sequenced K protein mRNA that was isolated from colorectal cancers as well as from normal tissues surrounding the tumours. Sequencing revealed a single G-to-A base substitution at position 274 that was found in tumours and surrounding mucosa, but not in individuals that had no colorectal tumour. This substitution most likely reflects an RNA editing event because it was not found in the corresponding genomic DNAs. Sequencing of RNA from normal colonic mucosa of patients with prior resection of colorectal cancer revealed only the wild-type K protein transcript, indicating that G274A isoform is tumour related. To our knowledge, this is the first example of an RNA editing event in cancer and its surrounding tissue, a finding that may offer a new diagnostic and treatment marker. Nature Publishing Group 2006-02-27 2006-01-10 /pmc/articles/PMC2361188/ /pubmed/16404425 http://dx.doi.org/10.1038/sj.bjc.6602938 Text en Copyright © 2006 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Genetics and Genomics Klimek-Tomczak, K Mikula, M Dzwonek, A Paziewska, A Karczmarski, J Hennig, E Bujnicki, J M Brągoszewski, P Denisenko, O Bomsztyk, K Ostrowski, J Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title | Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title_full | Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title_fullStr | Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title_full_unstemmed | Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title_short | Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa |
title_sort | editing of hnrnp k protein mrna in colorectal adenocarcinoma and surrounding mucosa |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361188/ https://www.ncbi.nlm.nih.gov/pubmed/16404425 http://dx.doi.org/10.1038/sj.bjc.6602938 |
work_keys_str_mv | AT klimektomczakk editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT mikulam editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT dzwoneka editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT paziewskaa editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT karczmarskij editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT hennige editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT bujnickijm editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT bragoszewskip editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT denisenkoo editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT bomsztykk editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa AT ostrowskij editingofhnrnpkproteinmrnaincolorectaladenocarcinomaandsurroundingmucosa |