LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy

New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, diffi...

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Autores principales: Albeanu, Dinu F., Soucy, Edward, Sato, Tomokazu F., Meister, Markus, Murthy, Venkatesh N.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361193/
https://www.ncbi.nlm.nih.gov/pubmed/18478056
http://dx.doi.org/10.1371/journal.pone.0002146
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author Albeanu, Dinu F.
Soucy, Edward
Sato, Tomokazu F.
Meister, Markus
Murthy, Venkatesh N.
author_facet Albeanu, Dinu F.
Soucy, Edward
Sato, Tomokazu F.
Meister, Markus
Murthy, Venkatesh N.
author_sort Albeanu, Dinu F.
collection PubMed
description New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.
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spelling pubmed-23611932008-05-14 LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy Albeanu, Dinu F. Soucy, Edward Sato, Tomokazu F. Meister, Markus Murthy, Venkatesh N. PLoS One Research Article New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging. Public Library of Science 2008-05-14 /pmc/articles/PMC2361193/ /pubmed/18478056 http://dx.doi.org/10.1371/journal.pone.0002146 Text en Albeanu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Albeanu, Dinu F.
Soucy, Edward
Sato, Tomokazu F.
Meister, Markus
Murthy, Venkatesh N.
LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title_full LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title_fullStr LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title_full_unstemmed LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title_short LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
title_sort led arrays as cost effective and efficient light sources for widefield microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361193/
https://www.ncbi.nlm.nih.gov/pubmed/18478056
http://dx.doi.org/10.1371/journal.pone.0002146
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AT meistermarkus ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy
AT murthyvenkateshn ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy

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