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Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2)
Snai2-deficient cells are radiosensitive to DNA damage. The function of Snai2 in response to DNA damage seems to be critical for its function in normal development and cancer. Here, we applied a functional genomics approach that combined gene-expression profiling and computational molecular network...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361430/ https://www.ncbi.nlm.nih.gov/pubmed/18182996 http://dx.doi.org/10.1038/sj.bjc.6604084 |
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author | Pérez-Caro, M Bermejo-Rodríguez, C González-Herrero, I Sánchez-Beato, M Piris, M A Sánchez-García, I |
author_facet | Pérez-Caro, M Bermejo-Rodríguez, C González-Herrero, I Sánchez-Beato, M Piris, M A Sánchez-García, I |
author_sort | Pérez-Caro, M |
collection | PubMed |
description | Snai2-deficient cells are radiosensitive to DNA damage. The function of Snai2 in response to DNA damage seems to be critical for its function in normal development and cancer. Here, we applied a functional genomics approach that combined gene-expression profiling and computational molecular network analysis to obtain global dissection of the Snai2-dependent transcriptional response to DNA damage in primary mouse embryonic fibroblasts (MEFs), which undergo p53-dependent growth arrest in response to DNA damage. Although examination of the response showed that overall expression of p53 target gene expression patterns was similarly altered in both control and Snai2-deficient cells, we have identified and validated candidate Snai2 target genes linked to Snai2 gene function in response to DNA damage. This work defines for the first time the effect of Snai2 on p53 target genes in cells undergoing growth arrest, elucidates the Snai2-dependent molecular network induced by DNA damage, points to novel putative Snai2 targets, and suggest a mechanistic model, which has implications for cancer management. |
format | Text |
id | pubmed-2361430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23614302009-09-10 Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) Pérez-Caro, M Bermejo-Rodríguez, C González-Herrero, I Sánchez-Beato, M Piris, M A Sánchez-García, I Br J Cancer Genetics and Genomics Snai2-deficient cells are radiosensitive to DNA damage. The function of Snai2 in response to DNA damage seems to be critical for its function in normal development and cancer. Here, we applied a functional genomics approach that combined gene-expression profiling and computational molecular network analysis to obtain global dissection of the Snai2-dependent transcriptional response to DNA damage in primary mouse embryonic fibroblasts (MEFs), which undergo p53-dependent growth arrest in response to DNA damage. Although examination of the response showed that overall expression of p53 target gene expression patterns was similarly altered in both control and Snai2-deficient cells, we have identified and validated candidate Snai2 target genes linked to Snai2 gene function in response to DNA damage. This work defines for the first time the effect of Snai2 on p53 target genes in cells undergoing growth arrest, elucidates the Snai2-dependent molecular network induced by DNA damage, points to novel putative Snai2 targets, and suggest a mechanistic model, which has implications for cancer management. Nature Publishing Group 2008-01-29 2008-01-08 /pmc/articles/PMC2361430/ /pubmed/18182996 http://dx.doi.org/10.1038/sj.bjc.6604084 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Genetics and Genomics Pérez-Caro, M Bermejo-Rodríguez, C González-Herrero, I Sánchez-Beato, M Piris, M A Sánchez-García, I Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title | Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title_full | Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title_fullStr | Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title_full_unstemmed | Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title_short | Transcriptomal profiling of the cellular response to DNA damage mediated by Slug (Snai2) |
title_sort | transcriptomal profiling of the cellular response to dna damage mediated by slug (snai2) |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361430/ https://www.ncbi.nlm.nih.gov/pubmed/18182996 http://dx.doi.org/10.1038/sj.bjc.6604084 |
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