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Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse
Nuclear factor (NF)-κB/p65 regulates the transcription of a wide variety of genes involved in cell survival, invasion and metastasis. We characterised by immunohistochemistry the expression of NF-κB/p65 protein in six histologically normal prostate, 13 high-grade prostatic intraepithelial neoplasia...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361509/ https://www.ncbi.nlm.nih.gov/pubmed/16278667 http://dx.doi.org/10.1038/sj.bjc.6602851 |
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author | Domingo-Domenech, J Mellado, B Ferrer, B Truan, D Codony-Servat, J Sauleda, S Alcover, J Campo, E Gascon, P Rovira, A Ross, J S Fernández, P L Albanell, J |
author_facet | Domingo-Domenech, J Mellado, B Ferrer, B Truan, D Codony-Servat, J Sauleda, S Alcover, J Campo, E Gascon, P Rovira, A Ross, J S Fernández, P L Albanell, J |
author_sort | Domingo-Domenech, J |
collection | PubMed |
description | Nuclear factor (NF)-κB/p65 regulates the transcription of a wide variety of genes involved in cell survival, invasion and metastasis. We characterised by immunohistochemistry the expression of NF-κB/p65 protein in six histologically normal prostate, 13 high-grade prostatic intraepithelial neoplasia (PIN) and 86 prostate adenocarcinoma specimens. Nuclear localisation of p65 was used as a measure of NF-κB active state. Nuclear localisation of NF-κB was only seen in scattered basal cells in normal prostate glands. Prostatic intraepithelial neoplasias exhibited diffuse and strong cytoplasmic staining but no nuclear staining. In prostate adenocarcinomas, cytoplasmic NF-κB was detected in 57 (66.3%) specimens, and nuclear NF-κB (activated) in 47 (54.7%). Nuclear and cytoplasmic NF-κB staining was not correlated (P=0.19). By univariate analysis, nuclear localisation of NF-κB was associated with biochemical relapse (P=0.0009; log-rank test) while cytoplasmic expression did not. On multivariate analysis, serum preoperative prostate specific antigen (P=0.02), Gleason score (P=0.03) and nuclear NF-κB (P=0.002) were independent predictors of biochemical relapse. These results provide novel evidence for NF-κB/p65 nuclear translocation in the transition from PIN to prostate cancer. Our findings also indicate that nuclear localisation of NF-κB is an independent prognostic factor of biochemical relapse in prostate cancer. |
format | Text |
id | pubmed-2361509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23615092009-09-10 Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse Domingo-Domenech, J Mellado, B Ferrer, B Truan, D Codony-Servat, J Sauleda, S Alcover, J Campo, E Gascon, P Rovira, A Ross, J S Fernández, P L Albanell, J Br J Cancer Molecular Diagnostics Nuclear factor (NF)-κB/p65 regulates the transcription of a wide variety of genes involved in cell survival, invasion and metastasis. We characterised by immunohistochemistry the expression of NF-κB/p65 protein in six histologically normal prostate, 13 high-grade prostatic intraepithelial neoplasia (PIN) and 86 prostate adenocarcinoma specimens. Nuclear localisation of p65 was used as a measure of NF-κB active state. Nuclear localisation of NF-κB was only seen in scattered basal cells in normal prostate glands. Prostatic intraepithelial neoplasias exhibited diffuse and strong cytoplasmic staining but no nuclear staining. In prostate adenocarcinomas, cytoplasmic NF-κB was detected in 57 (66.3%) specimens, and nuclear NF-κB (activated) in 47 (54.7%). Nuclear and cytoplasmic NF-κB staining was not correlated (P=0.19). By univariate analysis, nuclear localisation of NF-κB was associated with biochemical relapse (P=0.0009; log-rank test) while cytoplasmic expression did not. On multivariate analysis, serum preoperative prostate specific antigen (P=0.02), Gleason score (P=0.03) and nuclear NF-κB (P=0.002) were independent predictors of biochemical relapse. These results provide novel evidence for NF-κB/p65 nuclear translocation in the transition from PIN to prostate cancer. Our findings also indicate that nuclear localisation of NF-κB is an independent prognostic factor of biochemical relapse in prostate cancer. Nature Publishing Group 2005-11-28 2005-11-08 /pmc/articles/PMC2361509/ /pubmed/16278667 http://dx.doi.org/10.1038/sj.bjc.6602851 Text en Copyright © 2005 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Domingo-Domenech, J Mellado, B Ferrer, B Truan, D Codony-Servat, J Sauleda, S Alcover, J Campo, E Gascon, P Rovira, A Ross, J S Fernández, P L Albanell, J Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title | Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title_full | Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title_fullStr | Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title_full_unstemmed | Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title_short | Activation of nuclear factor-κB in human prostate carcinogenesis and association to biochemical relapse |
title_sort | activation of nuclear factor-κb in human prostate carcinogenesis and association to biochemical relapse |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361509/ https://www.ncbi.nlm.nih.gov/pubmed/16278667 http://dx.doi.org/10.1038/sj.bjc.6602851 |
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