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Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells

We recently identified genes and molecular pathways related to radioresistance of oral squamous cell carcinoma (OSCC) using Affymetrix GeneChip. The current study focused on the association between one of the target genes, intercellular adhesion molecule 2 (ICAM2), and resistance to X-ray irradiatio...

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Autores principales: Ishigami, T, Uzawa, K, Fushimi, K, Saito, K, Kato, Y, Nakashima, D, Higo, M, Kouzu, Y, Bukawa, H, Kawata, T, Ito, H, Tanzawa, H
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361700/
https://www.ncbi.nlm.nih.gov/pubmed/18349842
http://dx.doi.org/10.1038/sj.bjc.6604290
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author Ishigami, T
Uzawa, K
Fushimi, K
Saito, K
Kato, Y
Nakashima, D
Higo, M
Kouzu, Y
Bukawa, H
Kawata, T
Ito, H
Tanzawa, H
author_facet Ishigami, T
Uzawa, K
Fushimi, K
Saito, K
Kato, Y
Nakashima, D
Higo, M
Kouzu, Y
Bukawa, H
Kawata, T
Ito, H
Tanzawa, H
author_sort Ishigami, T
collection PubMed
description We recently identified genes and molecular pathways related to radioresistance of oral squamous cell carcinoma (OSCC) using Affymetrix GeneChip. The current study focused on the association between one of the target genes, intercellular adhesion molecule 2 (ICAM2), and resistance to X-ray irradiation in OSCC cells, and evaluated the antitumor efficacy of combining ICAM2 small interfering RNA (siRNA) and X-ray irradiation. Downregulation of ICAM2 expression by siRNA enhanced radiosensitivity of OSCC cells with the increased apoptotic phenotype via phosphorylation (ser473) of AKT and activation of caspase-3. Moreover, overexpression of ICAM2 induced greater OSCC cell resistance to the X-ray irradiation with the radioresistance phenotype. These results suggested that ICAM2 silencing is closely related to sensitivity of OSCC cells to radiotherapy, and that ICAM2 may be an effective radiotherapeutic target for this disease.
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spelling pubmed-23617002009-09-10 Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells Ishigami, T Uzawa, K Fushimi, K Saito, K Kato, Y Nakashima, D Higo, M Kouzu, Y Bukawa, H Kawata, T Ito, H Tanzawa, H Br J Cancer Translational Therapeutics We recently identified genes and molecular pathways related to radioresistance of oral squamous cell carcinoma (OSCC) using Affymetrix GeneChip. The current study focused on the association between one of the target genes, intercellular adhesion molecule 2 (ICAM2), and resistance to X-ray irradiation in OSCC cells, and evaluated the antitumor efficacy of combining ICAM2 small interfering RNA (siRNA) and X-ray irradiation. Downregulation of ICAM2 expression by siRNA enhanced radiosensitivity of OSCC cells with the increased apoptotic phenotype via phosphorylation (ser473) of AKT and activation of caspase-3. Moreover, overexpression of ICAM2 induced greater OSCC cell resistance to the X-ray irradiation with the radioresistance phenotype. These results suggested that ICAM2 silencing is closely related to sensitivity of OSCC cells to radiotherapy, and that ICAM2 may be an effective radiotherapeutic target for this disease. Nature Publishing Group 2008-04-22 2008-03-18 /pmc/articles/PMC2361700/ /pubmed/18349842 http://dx.doi.org/10.1038/sj.bjc.6604290 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Translational Therapeutics
Ishigami, T
Uzawa, K
Fushimi, K
Saito, K
Kato, Y
Nakashima, D
Higo, M
Kouzu, Y
Bukawa, H
Kawata, T
Ito, H
Tanzawa, H
Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title_full Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title_fullStr Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title_full_unstemmed Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title_short Inhibition of ICAM2 induces radiosensitisation in oral squamous cell carcinoma cells
title_sort inhibition of icam2 induces radiosensitisation in oral squamous cell carcinoma cells
topic Translational Therapeutics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361700/
https://www.ncbi.nlm.nih.gov/pubmed/18349842
http://dx.doi.org/10.1038/sj.bjc.6604290
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