Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer

The Fanconi gene family has a role in DNA repair and inactivation of FANCF has been proposed as a mechanism of sensitisation to platinum chemotherapy. This study sought to confirm this hypothesis in cell lines and a large series of ovarian cancer samples. Promoter methylation was assessed by methyla...

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Autores principales: Lim, S L, Smith, P, Syed, N, Coens, C, Wong, H, van der Burg, M, Szlosarek, P, Crook, T, Green, J A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2008
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361708/
https://www.ncbi.nlm.nih.gov/pubmed/18414472
http://dx.doi.org/10.1038/sj.bjc.6604325
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author Lim, S L
Smith, P
Syed, N
Coens, C
Wong, H
van der Burg, M
Szlosarek, P
Crook, T
Green, J A
author_facet Lim, S L
Smith, P
Syed, N
Coens, C
Wong, H
van der Burg, M
Szlosarek, P
Crook, T
Green, J A
author_sort Lim, S L
collection PubMed
description The Fanconi gene family has a role in DNA repair and inactivation of FANCF has been proposed as a mechanism of sensitisation to platinum chemotherapy. This study sought to confirm this hypothesis in cell lines and a large series of ovarian cancer samples. Promoter methylation was assessed by methylation-sensitive polymerase chain reaction of FANCF in nine ovarian cancer cell lines and 74 ovarian cancer samples taken from patients entered on a trial of cisplatin-based chemotherapy. This study confirmed methylation-dependent silencing of FANCF in one out of nine ovarian cancer cell lines. Methylation of FANCF was demonstrated in 13.2% of 53 evaluable ovarian tumour samples. Progression-free survival gave an HR of 3.63 (95% CI: 1.54–8.54, P=0.0016) in favour of the unmethylated cases. There was no association with overall survival. This study does not support methylation-dependent silencing of FANCF as a mechanism of sensitisation to platinum-based chemotherapy in ovarian cancer.
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spelling pubmed-23617082009-09-10 Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer Lim, S L Smith, P Syed, N Coens, C Wong, H van der Burg, M Szlosarek, P Crook, T Green, J A Br J Cancer Genetics and Genomics The Fanconi gene family has a role in DNA repair and inactivation of FANCF has been proposed as a mechanism of sensitisation to platinum chemotherapy. This study sought to confirm this hypothesis in cell lines and a large series of ovarian cancer samples. Promoter methylation was assessed by methylation-sensitive polymerase chain reaction of FANCF in nine ovarian cancer cell lines and 74 ovarian cancer samples taken from patients entered on a trial of cisplatin-based chemotherapy. This study confirmed methylation-dependent silencing of FANCF in one out of nine ovarian cancer cell lines. Methylation of FANCF was demonstrated in 13.2% of 53 evaluable ovarian tumour samples. Progression-free survival gave an HR of 3.63 (95% CI: 1.54–8.54, P=0.0016) in favour of the unmethylated cases. There was no association with overall survival. This study does not support methylation-dependent silencing of FANCF as a mechanism of sensitisation to platinum-based chemotherapy in ovarian cancer. Nature Publishing Group 2008-04-22 2008-04-15 /pmc/articles/PMC2361708/ /pubmed/18414472 http://dx.doi.org/10.1038/sj.bjc.6604325 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics and Genomics
Lim, S L
Smith, P
Syed, N
Coens, C
Wong, H
van der Burg, M
Szlosarek, P
Crook, T
Green, J A
Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title_full Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title_fullStr Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title_full_unstemmed Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title_short Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer
title_sort promoter hypermethylation of fancf and outcome in advanced ovarian cancer
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361708/
https://www.ncbi.nlm.nih.gov/pubmed/18414472
http://dx.doi.org/10.1038/sj.bjc.6604325
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