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Detection of human cytomegalovirus genome and gene products in central nervous system tumours

Human cytomegalovirus (HCMV) genome and related proteins have been reported in a great proportion of malignant gliomas. However, these results are unexpected since HCMV is not known as an oncogenic virus. By immunohistochemistry (with an anti-IE1 monoclonal antibody) and in situ hybridisation (with...

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Autores principales: Sabatier, J, Uro-Coste, E, Pommepuy, I, Labrousse, F, Allart, S, Trémoulet, M, Delisle, M B, Brousset, P
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361882/
https://www.ncbi.nlm.nih.gov/pubmed/15700045
http://dx.doi.org/10.1038/sj.bjc.6602339
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author Sabatier, J
Uro-Coste, E
Pommepuy, I
Labrousse, F
Allart, S
Trémoulet, M
Delisle, M B
Brousset, P
author_facet Sabatier, J
Uro-Coste, E
Pommepuy, I
Labrousse, F
Allart, S
Trémoulet, M
Delisle, M B
Brousset, P
author_sort Sabatier, J
collection PubMed
description Human cytomegalovirus (HCMV) genome and related proteins have been reported in a great proportion of malignant gliomas. However, these results are unexpected since HCMV is not known as an oncogenic virus. By immunohistochemistry (with an anti-IE1 monoclonal antibody) and in situ hybridisation (with biotinylated DNA probes) on tissue microarrays and frozen sections, we investigated a French series of central nervous system (CNS) tumours, including 97 glioblastomas. In 10 cases of glioblastoma, rare astrocyte-like cells, admixed with tumour cells, stained positively for HCMV and in one case a doubtful staining of rare cells was noticed. This may indicate a reactivation of the virus under local immunosuppression but none of the cases of CNS tumours (n=132) contained HCMV genomes and/or proteins in a significant proportion of tumour cells. Our results strongly suggest that HCMV is unlikely to be implicated in the development of human malignant gliomas, at least in French cases.
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spelling pubmed-23618822009-09-10 Detection of human cytomegalovirus genome and gene products in central nervous system tumours Sabatier, J Uro-Coste, E Pommepuy, I Labrousse, F Allart, S Trémoulet, M Delisle, M B Brousset, P Br J Cancer Molecular Diagnostics Human cytomegalovirus (HCMV) genome and related proteins have been reported in a great proportion of malignant gliomas. However, these results are unexpected since HCMV is not known as an oncogenic virus. By immunohistochemistry (with an anti-IE1 monoclonal antibody) and in situ hybridisation (with biotinylated DNA probes) on tissue microarrays and frozen sections, we investigated a French series of central nervous system (CNS) tumours, including 97 glioblastomas. In 10 cases of glioblastoma, rare astrocyte-like cells, admixed with tumour cells, stained positively for HCMV and in one case a doubtful staining of rare cells was noticed. This may indicate a reactivation of the virus under local immunosuppression but none of the cases of CNS tumours (n=132) contained HCMV genomes and/or proteins in a significant proportion of tumour cells. Our results strongly suggest that HCMV is unlikely to be implicated in the development of human malignant gliomas, at least in French cases. Nature Publishing Group 2005-02-28 2005-02-08 /pmc/articles/PMC2361882/ /pubmed/15700045 http://dx.doi.org/10.1038/sj.bjc.6602339 Text en Copyright © 2005 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Sabatier, J
Uro-Coste, E
Pommepuy, I
Labrousse, F
Allart, S
Trémoulet, M
Delisle, M B
Brousset, P
Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title_full Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title_fullStr Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title_full_unstemmed Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title_short Detection of human cytomegalovirus genome and gene products in central nervous system tumours
title_sort detection of human cytomegalovirus genome and gene products in central nervous system tumours
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361882/
https://www.ncbi.nlm.nih.gov/pubmed/15700045
http://dx.doi.org/10.1038/sj.bjc.6602339
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