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Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas

The vascular endothelial growth factor (VEGF) has been shown to be strictly related to vascular permeability and endothelial cell growth under physiological and pathological conditions. In tumour development and progression, VEGF plays a pivotal role in the development of the tumoral vascular networ...

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Autores principales: Fontanini, G, Boldrini, L, Chinè, S, Pisaturo, F, Basolo, F, Calcinai, A, Lucchi, M, Mussi, A, Angeletti, C A, Bevilacqua, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362204/
https://www.ncbi.nlm.nih.gov/pubmed/9888482
http://dx.doi.org/10.1038/sj.bjc.6690058
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author Fontanini, G
Boldrini, L
Chinè, S
Pisaturo, F
Basolo, F
Calcinai, A
Lucchi, M
Mussi, A
Angeletti, C A
Bevilacqua, G
author_facet Fontanini, G
Boldrini, L
Chinè, S
Pisaturo, F
Basolo, F
Calcinai, A
Lucchi, M
Mussi, A
Angeletti, C A
Bevilacqua, G
author_sort Fontanini, G
collection PubMed
description The vascular endothelial growth factor (VEGF) has been shown to be strictly related to vascular permeability and endothelial cell growth under physiological and pathological conditions. In tumour development and progression, VEGF plays a pivotal role in the development of the tumoral vascular network, and useful information in the progression of human cancer can be obtained by analysing the vascular endothelial growth factor expression of the tumours. In this study, we investigated the vascular endothelial growth factor transcript expression in non-small-cell lung carcinomas to evaluate the significance of this factor in a group of cancers in which the vascular pattern has been shown to significantly affect progression. Surgical samples of 42 patients with NSCLC were studied using reverse transcription polymerase chain reaction (PCR) analysis and in situ hybridization. Thirty-three out of 42 cases (78.6%) showed VEGF transcript expression predominantly as transcripts for the secretory forms of VEGF (isoforms 121 and 165). In situ hybridization, performed on 24 out of 42 samples, showed that the VEGF transcript expression was in several cases present in the cytoplasm both of neoplastic and normal cells, even if the VEGF mRNA was less expressed in the corresponding non-tumoral part. The VEGF 121 expression was associated with hilar and/or mediastinal nodal involvement (P = 0.02), and, taken together, the VEGF isoforms were shown to significantly influence overall (P = 0.02) and disease-free survival (P = 0.03). As a regulator of tumour angiogenesis, VEGF may represent a useful indicator of progression and poor prognosis in non-small-cell lung carcinomas. © 1999 Cancer Research Campaign
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spelling pubmed-23622042009-09-10 Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas Fontanini, G Boldrini, L Chinè, S Pisaturo, F Basolo, F Calcinai, A Lucchi, M Mussi, A Angeletti, C A Bevilacqua, G Br J Cancer Regular Article The vascular endothelial growth factor (VEGF) has been shown to be strictly related to vascular permeability and endothelial cell growth under physiological and pathological conditions. In tumour development and progression, VEGF plays a pivotal role in the development of the tumoral vascular network, and useful information in the progression of human cancer can be obtained by analysing the vascular endothelial growth factor expression of the tumours. In this study, we investigated the vascular endothelial growth factor transcript expression in non-small-cell lung carcinomas to evaluate the significance of this factor in a group of cancers in which the vascular pattern has been shown to significantly affect progression. Surgical samples of 42 patients with NSCLC were studied using reverse transcription polymerase chain reaction (PCR) analysis and in situ hybridization. Thirty-three out of 42 cases (78.6%) showed VEGF transcript expression predominantly as transcripts for the secretory forms of VEGF (isoforms 121 and 165). In situ hybridization, performed on 24 out of 42 samples, showed that the VEGF transcript expression was in several cases present in the cytoplasm both of neoplastic and normal cells, even if the VEGF mRNA was less expressed in the corresponding non-tumoral part. The VEGF 121 expression was associated with hilar and/or mediastinal nodal involvement (P = 0.02), and, taken together, the VEGF isoforms were shown to significantly influence overall (P = 0.02) and disease-free survival (P = 0.03). As a regulator of tumour angiogenesis, VEGF may represent a useful indicator of progression and poor prognosis in non-small-cell lung carcinomas. © 1999 Cancer Research Campaign Nature Publishing Group 1999-01 /pmc/articles/PMC2362204/ /pubmed/9888482 http://dx.doi.org/10.1038/sj.bjc.6690058 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Fontanini, G
Boldrini, L
Chinè, S
Pisaturo, F
Basolo, F
Calcinai, A
Lucchi, M
Mussi, A
Angeletti, C A
Bevilacqua, G
Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title_full Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title_fullStr Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title_full_unstemmed Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title_short Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas
title_sort expression of vascular endothelial growth factor mrna in non-small-cell lung carcinomas
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362204/
https://www.ncbi.nlm.nih.gov/pubmed/9888482
http://dx.doi.org/10.1038/sj.bjc.6690058
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