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Lack of DMBT1 expression in oesophageal, gastric and colon cancers

Loss of sequences from human chromosome 10q has been reported in several different cancers. Recently, a second candidate tumour-suppressor gene, DMBT1, was identified in this chromosomal region. We studied the mRNA expression and homozygous deletion of this gene in human oesophageal, gastric and col...

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Autores principales: Mori, M, Shiraishi, T, Tanaka, S, Yamagata, M, Mafune, K, Tanaka, Y, Ueo, H, Barnard, G F, Sugimachi, K
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362205/
https://www.ncbi.nlm.nih.gov/pubmed/9888459
http://dx.doi.org/10.1038/sj.bjc.6690035
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author Mori, M
Shiraishi, T
Tanaka, S
Yamagata, M
Mafune, K
Tanaka, Y
Ueo, H
Barnard, G F
Sugimachi, K
author_facet Mori, M
Shiraishi, T
Tanaka, S
Yamagata, M
Mafune, K
Tanaka, Y
Ueo, H
Barnard, G F
Sugimachi, K
author_sort Mori, M
collection PubMed
description Loss of sequences from human chromosome 10q has been reported in several different cancers. Recently, a second candidate tumour-suppressor gene, DMBT1, was identified in this chromosomal region. We studied the mRNA expression and homozygous deletion of this gene in human oesophageal, gastric and colon cancers. Reverse transcriptase polymerase chain reaction (RT-PCR) amplification demonstrated that 23 (53.5%) of 43 oesophageal, 5 (12.5%) of 40 gastric, and 4 (16.7%) of 24 colorectal cancer cases showed an apparent reduction in DMBT1 mRNA in tumour tissues compared with paired normal tissues. Twelve out of 15 oesophageal cancer cell lines also showed no expression. We next studied homozygous deletions within the DMBT1 gene in oesophageal cancers by using duplex PCR. Consequently, it was recognized in five (11.6%) of the primary tumours and two (13.3%) of the cell lines. These findings suggest that DMBT1 may act as a tumour-suppressor gene not only in brain tumours but also in gastrointestinal cancers, especially in oesophageal cancers. © 1999 Cancer Research Campaign
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spelling pubmed-23622052009-09-10 Lack of DMBT1 expression in oesophageal, gastric and colon cancers Mori, M Shiraishi, T Tanaka, S Yamagata, M Mafune, K Tanaka, Y Ueo, H Barnard, G F Sugimachi, K Br J Cancer Regular Article Loss of sequences from human chromosome 10q has been reported in several different cancers. Recently, a second candidate tumour-suppressor gene, DMBT1, was identified in this chromosomal region. We studied the mRNA expression and homozygous deletion of this gene in human oesophageal, gastric and colon cancers. Reverse transcriptase polymerase chain reaction (RT-PCR) amplification demonstrated that 23 (53.5%) of 43 oesophageal, 5 (12.5%) of 40 gastric, and 4 (16.7%) of 24 colorectal cancer cases showed an apparent reduction in DMBT1 mRNA in tumour tissues compared with paired normal tissues. Twelve out of 15 oesophageal cancer cell lines also showed no expression. We next studied homozygous deletions within the DMBT1 gene in oesophageal cancers by using duplex PCR. Consequently, it was recognized in five (11.6%) of the primary tumours and two (13.3%) of the cell lines. These findings suggest that DMBT1 may act as a tumour-suppressor gene not only in brain tumours but also in gastrointestinal cancers, especially in oesophageal cancers. © 1999 Cancer Research Campaign Nature Publishing Group 1999-01 /pmc/articles/PMC2362205/ /pubmed/9888459 http://dx.doi.org/10.1038/sj.bjc.6690035 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Mori, M
Shiraishi, T
Tanaka, S
Yamagata, M
Mafune, K
Tanaka, Y
Ueo, H
Barnard, G F
Sugimachi, K
Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title_full Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title_fullStr Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title_full_unstemmed Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title_short Lack of DMBT1 expression in oesophageal, gastric and colon cancers
title_sort lack of dmbt1 expression in oesophageal, gastric and colon cancers
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362205/
https://www.ncbi.nlm.nih.gov/pubmed/9888459
http://dx.doi.org/10.1038/sj.bjc.6690035
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