Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas

The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequenc...

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Autores principales: Kishima, H, Shimizu, K, Tamura, K, Miyao, Y, Mabuchi, E, Tominaga, E, Matsuzaki, J, Hayakawa, T
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362207/
https://www.ncbi.nlm.nih.gov/pubmed/9888477
http://dx.doi.org/10.1038/sj.bjc.6690053
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author Kishima, H
Shimizu, K
Tamura, K
Miyao, Y
Mabuchi, E
Tominaga, E
Matsuzaki, J
Hayakawa, T
author_facet Kishima, H
Shimizu, K
Tamura, K
Miyao, Y
Mabuchi, E
Tominaga, E
Matsuzaki, J
Hayakawa, T
author_sort Kishima, H
collection PubMed
description The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin α3. When the integrin α3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin α3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin α3, might be useful in the diagnosis of gliomas and medulloblastomas. © 1999 Cancer Research Campaign
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spelling pubmed-23622072009-09-10 Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas Kishima, H Shimizu, K Tamura, K Miyao, Y Mabuchi, E Tominaga, E Matsuzaki, J Hayakawa, T Br J Cancer Regular Article The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin α3. When the integrin α3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin α3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin α3, might be useful in the diagnosis of gliomas and medulloblastomas. © 1999 Cancer Research Campaign Nature Publishing Group 1999-01 /pmc/articles/PMC2362207/ /pubmed/9888477 http://dx.doi.org/10.1038/sj.bjc.6690053 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Kishima, H
Shimizu, K
Tamura, K
Miyao, Y
Mabuchi, E
Tominaga, E
Matsuzaki, J
Hayakawa, T
Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title_full Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title_fullStr Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title_full_unstemmed Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title_short Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas
title_sort monoclonal antibody ons-m21 recognizes integrin α3 in gliomas and medulloblastomas
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362207/
https://www.ncbi.nlm.nih.gov/pubmed/9888477
http://dx.doi.org/10.1038/sj.bjc.6690053
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