Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin

We have investigated the production, growth and inactivation of gastrin-releasing peptide (GRP)-like peptides in human breast cancer cell lines. Radioimmunoassay detected GRP-like immunoreactivity (GRP-LI) in T47D breast cancer cells but not in the conditioned medium, indicating rapid clearance. No...

Descripción completa

Detalles Bibliográficos
Autores principales: Burns, D M, Walker, B, Gray, J, Nelson, J
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362209/
https://www.ncbi.nlm.nih.gov/pubmed/9888460
http://dx.doi.org/10.1038/sj.bjc.6690036
_version_ 1782153400836685824
author Burns, D M
Walker, B
Gray, J
Nelson, J
author_facet Burns, D M
Walker, B
Gray, J
Nelson, J
author_sort Burns, D M
collection PubMed
description We have investigated the production, growth and inactivation of gastrin-releasing peptide (GRP)-like peptides in human breast cancer cell lines. Radioimmunoassay detected GRP-like immunoreactivity (GRP-LI) in T47D breast cancer cells but not in the conditioned medium, indicating rapid clearance. No GRP-LI was found in the ZR-75-1 or MDA-MB-436 cells or their conditioned medium. High-performance liquid chromatography (HPLC) analysis of the GRP-LI in the T47D cells revealed a major peak, which co-eluted with GRP(18–27), and a minor more hydrophilic peak. In vitro stimulation of T47D cell growth by bombesin (BN) was enhanced to 138% of control levels (bombesin alone) by the addition of the selective endopeptidase EC 3.4.24.11 inhibitor phosphoramidon (0.1 ng ml(−;1)). Fluorogenic analysis using whole cells confirmed low levels of this phosphoramidon-sensitive enzyme on the T47D cells. This enzyme, previously unreported in human breast cancer cells, significantly modulates both T47D growth and its response to BN-induced growth. © 1999 Cancer Research Campaign
format Text
id pubmed-2362209
institution National Center for Biotechnology Information
language English
publishDate 1999
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23622092009-09-10 Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin Burns, D M Walker, B Gray, J Nelson, J Br J Cancer Regular Article We have investigated the production, growth and inactivation of gastrin-releasing peptide (GRP)-like peptides in human breast cancer cell lines. Radioimmunoassay detected GRP-like immunoreactivity (GRP-LI) in T47D breast cancer cells but not in the conditioned medium, indicating rapid clearance. No GRP-LI was found in the ZR-75-1 or MDA-MB-436 cells or their conditioned medium. High-performance liquid chromatography (HPLC) analysis of the GRP-LI in the T47D cells revealed a major peak, which co-eluted with GRP(18–27), and a minor more hydrophilic peak. In vitro stimulation of T47D cell growth by bombesin (BN) was enhanced to 138% of control levels (bombesin alone) by the addition of the selective endopeptidase EC 3.4.24.11 inhibitor phosphoramidon (0.1 ng ml(−;1)). Fluorogenic analysis using whole cells confirmed low levels of this phosphoramidon-sensitive enzyme on the T47D cells. This enzyme, previously unreported in human breast cancer cells, significantly modulates both T47D growth and its response to BN-induced growth. © 1999 Cancer Research Campaign Nature Publishing Group 1999-01 /pmc/articles/PMC2362209/ /pubmed/9888460 http://dx.doi.org/10.1038/sj.bjc.6690036 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Burns, D M
Walker, B
Gray, J
Nelson, J
Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title_full Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title_fullStr Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title_full_unstemmed Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title_short Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin
title_sort breast cancer cell-associated endopeptidase ec 24.11 modulates proliferative response to bombesin
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362209/
https://www.ncbi.nlm.nih.gov/pubmed/9888460
http://dx.doi.org/10.1038/sj.bjc.6690036
work_keys_str_mv AT burnsdm breastcancercellassociatedendopeptidaseec2411modulatesproliferativeresponsetobombesin
AT walkerb breastcancercellassociatedendopeptidaseec2411modulatesproliferativeresponsetobombesin
AT grayj breastcancercellassociatedendopeptidaseec2411modulatesproliferativeresponsetobombesin
AT nelsonj breastcancercellassociatedendopeptidaseec2411modulatesproliferativeresponsetobombesin

Ejemplares similares