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In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data

The aim was to characterize the variation in the cellular in vitro radiosensitivities in squamous cell carcinomas of the head and neck, and to test for a possible correlation between different measures of radiosensitivity and the clinical and histopathological data. Cellular in vitro radiosensitivit...

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Autores principales: Stausbøl-Grøn, B, Bentzen, S M, Jørgensen, K E, Nielsen, O S, Bundgaard, T, Overgaard, J
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362224/
https://www.ncbi.nlm.nih.gov/pubmed/10098739
http://dx.doi.org/10.1038/sj.bjc.6690172
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author Stausbøl-Grøn, B
Bentzen, S M
Jørgensen, K E
Nielsen, O S
Bundgaard, T
Overgaard, J
author_facet Stausbøl-Grøn, B
Bentzen, S M
Jørgensen, K E
Nielsen, O S
Bundgaard, T
Overgaard, J
author_sort Stausbøl-Grøn, B
collection PubMed
description The aim was to characterize the variation in the cellular in vitro radiosensitivities in squamous cell carcinomas of the head and neck, and to test for a possible correlation between different measures of radiosensitivity and the clinical and histopathological data. Cellular in vitro radiosensitivities were assessed in tumour biopsies from 71 patients using the modified Courtenay–Mills soft agar clonogenic assay combined with an immunocytochemical analysis. Radiosensitivity was quantified as the surviving fraction after a radiation dose of 2 Gy irrespective of cell type (overall SF(2)), or based on identification of cell type (tumour cell SF(2), fibroblast SF(2)). Sixty-three biopsies were from primary tumours, and eight were from recurrences. Overall plating efficiency ranged from 0.005 to 1.60% with a median of 0.052%. The majority of the colonies obtained from the biopsies were fibroblast marker-positive; the proportion of tumour marker-positive colonies ranged from 1 to 88% with a median of 15%. The median overall SF(2) was 0.47 (range 0.24–0.96), the median tumour cell SF(2) was 0.50 (range 0.11–1.0) and the median fibroblast SF(2) was 0.49 (range 0.24–1.0). Comparing data from independent experiments, the overall SF(2) was significantly correlated with the SF(2) of fibroblasts (2P = 0.006) but not with the tumour cell SF(2). The tumour cell and fibroblast radiosensitivities measured in the same individuals were not correlated (r = 0.06, 95% CI [–0.19, 0.30]). This finding seems to preclude a strong correlation between the radiosensitivity of tumour cells and fibroblasts. Concerning the clinical characteristics, neither of the measures of tumour radiosensitivity was correlated with T- and N-category, stage, tumour size, sex and age. However, the tumour cell radiosensitivity decreased with increasing grade of histopathological differentiation (2P = 0.012). The same tendency was found in two independent analyses of the same patient material. This correlation was not significant in case of the overall SF(2) or the fibroblast SF(2). © 1999 Cancer Research Campaign
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spelling pubmed-23622242009-09-10 In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data Stausbøl-Grøn, B Bentzen, S M Jørgensen, K E Nielsen, O S Bundgaard, T Overgaard, J Br J Cancer Regular Article The aim was to characterize the variation in the cellular in vitro radiosensitivities in squamous cell carcinomas of the head and neck, and to test for a possible correlation between different measures of radiosensitivity and the clinical and histopathological data. Cellular in vitro radiosensitivities were assessed in tumour biopsies from 71 patients using the modified Courtenay–Mills soft agar clonogenic assay combined with an immunocytochemical analysis. Radiosensitivity was quantified as the surviving fraction after a radiation dose of 2 Gy irrespective of cell type (overall SF(2)), or based on identification of cell type (tumour cell SF(2), fibroblast SF(2)). Sixty-three biopsies were from primary tumours, and eight were from recurrences. Overall plating efficiency ranged from 0.005 to 1.60% with a median of 0.052%. The majority of the colonies obtained from the biopsies were fibroblast marker-positive; the proportion of tumour marker-positive colonies ranged from 1 to 88% with a median of 15%. The median overall SF(2) was 0.47 (range 0.24–0.96), the median tumour cell SF(2) was 0.50 (range 0.11–1.0) and the median fibroblast SF(2) was 0.49 (range 0.24–1.0). Comparing data from independent experiments, the overall SF(2) was significantly correlated with the SF(2) of fibroblasts (2P = 0.006) but not with the tumour cell SF(2). The tumour cell and fibroblast radiosensitivities measured in the same individuals were not correlated (r = 0.06, 95% CI [–0.19, 0.30]). This finding seems to preclude a strong correlation between the radiosensitivity of tumour cells and fibroblasts. Concerning the clinical characteristics, neither of the measures of tumour radiosensitivity was correlated with T- and N-category, stage, tumour size, sex and age. However, the tumour cell radiosensitivity decreased with increasing grade of histopathological differentiation (2P = 0.012). The same tendency was found in two independent analyses of the same patient material. This correlation was not significant in case of the overall SF(2) or the fibroblast SF(2). © 1999 Cancer Research Campaign Nature Publishing Group 1999-03 /pmc/articles/PMC2362224/ /pubmed/10098739 http://dx.doi.org/10.1038/sj.bjc.6690172 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Stausbøl-Grøn, B
Bentzen, S M
Jørgensen, K E
Nielsen, O S
Bundgaard, T
Overgaard, J
In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title_full In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title_fullStr In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title_full_unstemmed In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title_short In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
title_sort in vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362224/
https://www.ncbi.nlm.nih.gov/pubmed/10098739
http://dx.doi.org/10.1038/sj.bjc.6690172
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