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FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas

Colorectal carcinoma cells have recently been shown to express Fas ligand (FasL). This ligand could allow the tumour cells to evade activated tumour-infiltrating lymphocytes (TILs) by inducing their apoptosis and would thus promote tumour survival and possibly metastasis formation. To test this hypo...

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Autores principales: Mann, B, Gratchev, A, Böhm, C, Demel, G, Trojanek, B, Schmidt-Wolf, I, Stein, H, Hanski, C
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362258/
https://www.ncbi.nlm.nih.gov/pubmed/10098769
http://dx.doi.org/10.1038/sj.bjc.6690202
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author Mann, B
Gratchev, A
Böhm, C
Demel, G
Trojanek, B
Schmidt-Wolf, I
Stein, H
Hanski, C
author_facet Mann, B
Gratchev, A
Böhm, C
Demel, G
Trojanek, B
Schmidt-Wolf, I
Stein, H
Hanski, C
author_sort Mann, B
collection PubMed
description Colorectal carcinoma cells have recently been shown to express Fas ligand (FasL). This ligand could allow the tumour cells to evade activated tumour-infiltrating lymphocytes (TILs) by inducing their apoptosis and would thus promote tumour survival and possibly metastasis formation. To test this hypothesis in vivo we analysed the expression of FasL mRNA and protein in paired tissue samples of normal colonic mucosa (N), primary colorectal carcinomas (T) and their metastases (M) from a total of 21 patients by four different methods. Additionally, the presence and activation status of infiltrating lymphocytes, which might contribute to the total amount of FasL in the tissue, was determined by semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) in the same samples. The frequency of FasL detection was 30–40% in T and was 60–100% in M, depending on the sensitivity of the method. Simultaneously, the amount of CD25 mRNA, used as a measure of the number of activated TILs, was in 90% of patients lower in M than in T. The increased frequency of FasL detection in liver metastases was therefore not due to the presence of activated TILs. We conclude that metastasizing subpopulations of colorectal tumour cells express FasL more frequently than the primary carcinomas and may be able to eliminate activated TILs in vivo via Fas/FasL-induced apoptosis or other hitherto unknown mechanisms. © 1999 Cancer Research Campaign
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spelling pubmed-23622582009-09-10 FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas Mann, B Gratchev, A Böhm, C Demel, G Trojanek, B Schmidt-Wolf, I Stein, H Hanski, C Br J Cancer Regular Article Colorectal carcinoma cells have recently been shown to express Fas ligand (FasL). This ligand could allow the tumour cells to evade activated tumour-infiltrating lymphocytes (TILs) by inducing their apoptosis and would thus promote tumour survival and possibly metastasis formation. To test this hypothesis in vivo we analysed the expression of FasL mRNA and protein in paired tissue samples of normal colonic mucosa (N), primary colorectal carcinomas (T) and their metastases (M) from a total of 21 patients by four different methods. Additionally, the presence and activation status of infiltrating lymphocytes, which might contribute to the total amount of FasL in the tissue, was determined by semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) in the same samples. The frequency of FasL detection was 30–40% in T and was 60–100% in M, depending on the sensitivity of the method. Simultaneously, the amount of CD25 mRNA, used as a measure of the number of activated TILs, was in 90% of patients lower in M than in T. The increased frequency of FasL detection in liver metastases was therefore not due to the presence of activated TILs. We conclude that metastasizing subpopulations of colorectal tumour cells express FasL more frequently than the primary carcinomas and may be able to eliminate activated TILs in vivo via Fas/FasL-induced apoptosis or other hitherto unknown mechanisms. © 1999 Cancer Research Campaign Nature Publishing Group 1999-03 /pmc/articles/PMC2362258/ /pubmed/10098769 http://dx.doi.org/10.1038/sj.bjc.6690202 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Mann, B
Gratchev, A
Böhm, C
Demel, G
Trojanek, B
Schmidt-Wolf, I
Stein, H
Hanski, C
FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title_full FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title_fullStr FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title_full_unstemmed FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title_short FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
title_sort fasl is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362258/
https://www.ncbi.nlm.nih.gov/pubmed/10098769
http://dx.doi.org/10.1038/sj.bjc.6690202
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