Cargando…
Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid
All nucleated mammalian cells synthesize protoporphyrin IX (PpIX) when exposed to exogenous 5-aminolevulinic acid (ALA). The response to exogenous ALA under standard conditions (the ALA phenotype) is characteristic for each cell type. Significantly more PpIX accumulates in malignant and premalignant...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1999
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362266/ https://www.ncbi.nlm.nih.gov/pubmed/10360643 http://dx.doi.org/10.1038/sj.bjc.6690409 |
_version_ | 1782153415117242368 |
---|---|
author | Li, G Szewczuk, M R Raptis, L Johnson, J G Weagle, G E Pottier, R H Kennedy, J C |
author_facet | Li, G Szewczuk, M R Raptis, L Johnson, J G Weagle, G E Pottier, R H Kennedy, J C |
author_sort | Li, G |
collection | PubMed |
description | All nucleated mammalian cells synthesize protoporphyrin IX (PpIX) when exposed to exogenous 5-aminolevulinic acid (ALA). The response to exogenous ALA under standard conditions (the ALA phenotype) is characteristic for each cell type. Significantly more PpIX accumulates in malignant and premalignant cells than in the normal cells from which they were derived. A rodent fibroblast model was developed to study the mechanisms responsible for this phenomenon. Exogenous ALA induced the accumulation of substantial concentrations of PpIX in fibrosarcoma cells, and in immortalized fibroblasts transfected with the oncogene c-myc, IGF-1 receptor, IGF-1 and its receptor, v-fos, v-raf, v-Ki-ras, v-abl, or polyomavirus middle T antigen with G418 resistance selection. Much lower concentrations of PpIX accumulated in primary fibroblast cultures, in immortalized fibroblast cell lines, and in immortalized fibroblasts transfected with the G418-resistance gene only. The mechanisms responsible for the increased accumulation of ALA-induced PpIX by transformed cells (the malignant ALA phenotype) therefore appear to be closely linked to the mechanisms responsible for malignant transformation. Identification of the nature of that linkage may lead to new approaches to cancer therapy. © 1999 Cancer Research Campaign |
format | Text |
id | pubmed-2362266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23622662009-09-10 Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid Li, G Szewczuk, M R Raptis, L Johnson, J G Weagle, G E Pottier, R H Kennedy, J C Br J Cancer Regular Article All nucleated mammalian cells synthesize protoporphyrin IX (PpIX) when exposed to exogenous 5-aminolevulinic acid (ALA). The response to exogenous ALA under standard conditions (the ALA phenotype) is characteristic for each cell type. Significantly more PpIX accumulates in malignant and premalignant cells than in the normal cells from which they were derived. A rodent fibroblast model was developed to study the mechanisms responsible for this phenomenon. Exogenous ALA induced the accumulation of substantial concentrations of PpIX in fibrosarcoma cells, and in immortalized fibroblasts transfected with the oncogene c-myc, IGF-1 receptor, IGF-1 and its receptor, v-fos, v-raf, v-Ki-ras, v-abl, or polyomavirus middle T antigen with G418 resistance selection. Much lower concentrations of PpIX accumulated in primary fibroblast cultures, in immortalized fibroblast cell lines, and in immortalized fibroblasts transfected with the G418-resistance gene only. The mechanisms responsible for the increased accumulation of ALA-induced PpIX by transformed cells (the malignant ALA phenotype) therefore appear to be closely linked to the mechanisms responsible for malignant transformation. Identification of the nature of that linkage may lead to new approaches to cancer therapy. © 1999 Cancer Research Campaign Nature Publishing Group 1999-05 /pmc/articles/PMC2362266/ /pubmed/10360643 http://dx.doi.org/10.1038/sj.bjc.6690409 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Li, G Szewczuk, M R Raptis, L Johnson, J G Weagle, G E Pottier, R H Kennedy, J C Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title | Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title_full | Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title_fullStr | Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title_full_unstemmed | Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title_short | Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
title_sort | rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362266/ https://www.ncbi.nlm.nih.gov/pubmed/10360643 http://dx.doi.org/10.1038/sj.bjc.6690409 |
work_keys_str_mv | AT lig rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT szewczukmr rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT raptisl rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT johnsonjg rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT weaglege rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT pottierrh rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid AT kennedyjc rodentfibroblastmodelforstudiesofresponseofmalignantcellstoexogenous5aminolevulinicacid |