Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan

Elucidation of the basic genetic changes of human hepatocellular carcinoma is important for the understanding and treatment of this cancer. We used microsatellite polymorphism markers to study 30 cases of hepatocellular carcinoma (34 tumours) on all human chromosomes. DNA from 34 pairs of hepatocell...

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Autores principales: Sheu, J-C, Lin, Y-W, Chou, H-C, Huang, G-T, Lee, H-S, Lin, Y-H, Huang, S-Y, Chen, C-H, Wang, J-T, Lee, P-H, Lin, J-T, Lu, F-J, Chen, D-S
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362334/
https://www.ncbi.nlm.nih.gov/pubmed/10408855
http://dx.doi.org/10.1038/sj.bjc.6690380
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author Sheu, J-C
Lin, Y-W
Chou, H-C
Huang, G-T
Lee, H-S
Lin, Y-H
Huang, S-Y
Chen, C-H
Wang, J-T
Lee, P-H
Lin, J-T
Lu, F-J
Chen, D-S
author_facet Sheu, J-C
Lin, Y-W
Chou, H-C
Huang, G-T
Lee, H-S
Lin, Y-H
Huang, S-Y
Chen, C-H
Wang, J-T
Lee, P-H
Lin, J-T
Lu, F-J
Chen, D-S
author_sort Sheu, J-C
collection PubMed
description Elucidation of the basic genetic changes of human hepatocellular carcinoma is important for the understanding and treatment of this cancer. We used microsatellite polymorphism markers to study 30 cases of hepatocellular carcinoma (34 tumours) on all human chromosomes. DNA from 34 pairs of hepatocellular carcinomas and corresponding non-tumour parts was prepared. Loss of heterozygosity (LOH) and microsatellite instability on 23 chromosomes were investigated by 231 sets of microsatellite markers. More than 20% LOH was shown for loci on 16q (47.1%), 13q (32.4%), 17p (32.4%), 5q (26.5%), 11p (23.5%) and 9p (20.6%). The commonly affected regions were mapped to 16q12.1, 16q12.2, 16q24, 13q12.1–32, 17p13, 5q32, 5q34, 5q3, 11p15, 11q23–24 and 9p21. Hepatitis B virus carriers had a significantly higher frequency of LOH on chromosomes 5q, 11p and 16q. Furthermore, larger tumour size tended to have higher frequency of LOH at D16S409 locus (16q12.1). Microsatellte instability was only found in 12 of 231 markers and the frequency is very low. These data suggest that the chromosomes 16q, 13q, 17p, 5q, 11p and 9p might participate in hepatocarcinogenesis. However, microsatellite instability might play little role in the development of this cancer in Taiwan. © 1999 Cancer Research Campaign
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spelling pubmed-23623342009-09-10 Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan Sheu, J-C Lin, Y-W Chou, H-C Huang, G-T Lee, H-S Lin, Y-H Huang, S-Y Chen, C-H Wang, J-T Lee, P-H Lin, J-T Lu, F-J Chen, D-S Br J Cancer Regular Article Elucidation of the basic genetic changes of human hepatocellular carcinoma is important for the understanding and treatment of this cancer. We used microsatellite polymorphism markers to study 30 cases of hepatocellular carcinoma (34 tumours) on all human chromosomes. DNA from 34 pairs of hepatocellular carcinomas and corresponding non-tumour parts was prepared. Loss of heterozygosity (LOH) and microsatellite instability on 23 chromosomes were investigated by 231 sets of microsatellite markers. More than 20% LOH was shown for loci on 16q (47.1%), 13q (32.4%), 17p (32.4%), 5q (26.5%), 11p (23.5%) and 9p (20.6%). The commonly affected regions were mapped to 16q12.1, 16q12.2, 16q24, 13q12.1–32, 17p13, 5q32, 5q34, 5q3, 11p15, 11q23–24 and 9p21. Hepatitis B virus carriers had a significantly higher frequency of LOH on chromosomes 5q, 11p and 16q. Furthermore, larger tumour size tended to have higher frequency of LOH at D16S409 locus (16q12.1). Microsatellte instability was only found in 12 of 231 markers and the frequency is very low. These data suggest that the chromosomes 16q, 13q, 17p, 5q, 11p and 9p might participate in hepatocarcinogenesis. However, microsatellite instability might play little role in the development of this cancer in Taiwan. © 1999 Cancer Research Campaign Nature Publishing Group 1999-05 1999-05-01 /pmc/articles/PMC2362334/ /pubmed/10408855 http://dx.doi.org/10.1038/sj.bjc.6690380 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Sheu, J-C
Lin, Y-W
Chou, H-C
Huang, G-T
Lee, H-S
Lin, Y-H
Huang, S-Y
Chen, C-H
Wang, J-T
Lee, P-H
Lin, J-T
Lu, F-J
Chen, D-S
Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title_full Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title_fullStr Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title_full_unstemmed Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title_short Loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in Taiwan
title_sort loss of heterozygosity and microsatellite instability in hepatocellular carcinoma in taiwan
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362334/
https://www.ncbi.nlm.nih.gov/pubmed/10408855
http://dx.doi.org/10.1038/sj.bjc.6690380
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