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Development of a sensitive, specific reverse transcriptase polymerase chain reaction-based assay for epithelial tumour cells in effusions
We developed a sensitive and specific method for the detection of epithelial cancer cells in effusions with a two-stage molecular-based assay which combined enrichment for cancer cells by immunomagnetic bead selection and reverse transcriptase polymerase chain reaction (RT-PCR) detection of epitheli...
Autores principales: | Sakaguchi, M, Virmani, A K, Ashfaq, R, Rogers, T E, Rathi, A, Liu, Y, Kodagoda, D, Cunningham, H T, Gazdar, A F |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362411/ https://www.ncbi.nlm.nih.gov/pubmed/10027307 http://dx.doi.org/10.1038/sj.bjc.6690065 |
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