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The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines

The contribution of DNA ploidy to radiation sensitivity was investigated in a group of eight human tumour cell lines. As previous studies suggest, while more aneuploid tumours tend to be more radioresistant, there is no significant relationship between ploidy and radiation sensitivity (SF(2)). The f...

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Autores principales: Schwartz, J L, Murnane, J, Weichselbaum, R R
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362676/
https://www.ncbi.nlm.nih.gov/pubmed/10070863
http://dx.doi.org/10.1038/sj.bjc.6690119
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author Schwartz, J L
Murnane, J
Weichselbaum, R R
author_facet Schwartz, J L
Murnane, J
Weichselbaum, R R
author_sort Schwartz, J L
collection PubMed
description The contribution of DNA ploidy to radiation sensitivity was investigated in a group of eight human tumour cell lines. As previous studies suggest, while more aneuploid tumours tend to be more radioresistant, there is no significant relationship between ploidy and radiation sensitivity (SF(2)). The failure to observe a significant effect of ploidy on radiation sensitivity is due to the complex and multifactorial basis of radiation sensitivity. When we determined the relationship between survival and radiation-induced chromosome aberration frequency, a measure independent of most other modifiers of sensitivity, we observed a direct relationship between ploidy and mean lethal aberration frequency. The mean lethal frequency of aberrations increased from about 1 for diploid cells to about 2 for tetraploid cells. The mean lethal frequency of aberrations was independent of DNA repair variations. These observations demonstrate that changes in DNA ploidy are an important contributor to radiation sensitivity variations in human tumour cell lines. Therefore, any battery of predictive assays should include DNA ploidy measurements. © 1999 Cancer Research Campaign
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spelling pubmed-23626762009-09-10 The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines Schwartz, J L Murnane, J Weichselbaum, R R Br J Cancer Regular Article The contribution of DNA ploidy to radiation sensitivity was investigated in a group of eight human tumour cell lines. As previous studies suggest, while more aneuploid tumours tend to be more radioresistant, there is no significant relationship between ploidy and radiation sensitivity (SF(2)). The failure to observe a significant effect of ploidy on radiation sensitivity is due to the complex and multifactorial basis of radiation sensitivity. When we determined the relationship between survival and radiation-induced chromosome aberration frequency, a measure independent of most other modifiers of sensitivity, we observed a direct relationship between ploidy and mean lethal aberration frequency. The mean lethal frequency of aberrations increased from about 1 for diploid cells to about 2 for tetraploid cells. The mean lethal frequency of aberrations was independent of DNA repair variations. These observations demonstrate that changes in DNA ploidy are an important contributor to radiation sensitivity variations in human tumour cell lines. Therefore, any battery of predictive assays should include DNA ploidy measurements. © 1999 Cancer Research Campaign Nature Publishing Group 1999-02 /pmc/articles/PMC2362676/ /pubmed/10070863 http://dx.doi.org/10.1038/sj.bjc.6690119 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Schwartz, J L
Murnane, J
Weichselbaum, R R
The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title_full The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title_fullStr The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title_full_unstemmed The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title_short The contribution of DNA ploidy to radiation sensitivity in human tumour cell lines
title_sort contribution of dna ploidy to radiation sensitivity in human tumour cell lines
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362676/
https://www.ncbi.nlm.nih.gov/pubmed/10070863
http://dx.doi.org/10.1038/sj.bjc.6690119
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