Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel

Berberine is the major constituent of Coptis chinese and is commonly used in Chinese herbal medicine to treat patients with gastrointestinal disorders. In this study, using flow cytometry, we have found that a 24-h berberine treatment up-regulated the multidrug-resistant transporter (pgp-170) expres...

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Autores principales: Lin, H-L, Liu, T-Y, Wu, C-W, Chi, C-W
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362909/
https://www.ncbi.nlm.nih.gov/pubmed/10507765
http://dx.doi.org/10.1038/sj.bjc.6690710
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author Lin, H-L
Liu, T-Y
Wu, C-W
Chi, C-W
author_facet Lin, H-L
Liu, T-Y
Wu, C-W
Chi, C-W
author_sort Lin, H-L
collection PubMed
description Berberine is the major constituent of Coptis chinese and is commonly used in Chinese herbal medicine to treat patients with gastrointestinal disorders. In this study, using flow cytometry, we have found that a 24-h berberine treatment up-regulated the multidrug-resistant transporter (pgp-170) expression in two oral (KB, OC2), two gastric (SC-M1, NUGC-3) and two colon (COLO 205, CT 26) cancer cell lines. Decreased retention of rhodamine 123 was observed in berberine-treated cells as compared to vehicle control. To examine whether the berberine modulated pgp-170 expression in cancer cells is associated with changes in drug resistance, we determined the cytotoxicity, cell cycle progression and cell morphology of Paclitaxel-treated cells. Paclitaxel (1 nM–10 μM) treatment for 24 h induced cytotoxicity in OC2, SC-M1 and COLO 205 cells in a dose-dependent manner. Pretreatment of cells with 32 μM berberine for 24 h prior to Paclitaxel treatment resulted in increased viability as compared to that of Paclitaxel-treated cells. In addition, Paclitaxel-induced apoptosis and/or G2/M arrest in these three cancer cell lines. Pretreatment of cells with berberine prior to Paclitaxel blocked the Paclitaxel-induced cell cycle responses and morphological changes. These results together suggest that berberine modulated the expression and function of pgp-170 that leads to reduced response to Paclitaxel in digestive track cancer cells. © 1999 Cancer Research Campaign
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spelling pubmed-23629092009-09-10 Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel Lin, H-L Liu, T-Y Wu, C-W Chi, C-W Br J Cancer Regular Article Berberine is the major constituent of Coptis chinese and is commonly used in Chinese herbal medicine to treat patients with gastrointestinal disorders. In this study, using flow cytometry, we have found that a 24-h berberine treatment up-regulated the multidrug-resistant transporter (pgp-170) expression in two oral (KB, OC2), two gastric (SC-M1, NUGC-3) and two colon (COLO 205, CT 26) cancer cell lines. Decreased retention of rhodamine 123 was observed in berberine-treated cells as compared to vehicle control. To examine whether the berberine modulated pgp-170 expression in cancer cells is associated with changes in drug resistance, we determined the cytotoxicity, cell cycle progression and cell morphology of Paclitaxel-treated cells. Paclitaxel (1 nM–10 μM) treatment for 24 h induced cytotoxicity in OC2, SC-M1 and COLO 205 cells in a dose-dependent manner. Pretreatment of cells with 32 μM berberine for 24 h prior to Paclitaxel treatment resulted in increased viability as compared to that of Paclitaxel-treated cells. In addition, Paclitaxel-induced apoptosis and/or G2/M arrest in these three cancer cell lines. Pretreatment of cells with berberine prior to Paclitaxel blocked the Paclitaxel-induced cell cycle responses and morphological changes. These results together suggest that berberine modulated the expression and function of pgp-170 that leads to reduced response to Paclitaxel in digestive track cancer cells. © 1999 Cancer Research Campaign Nature Publishing Group 1999-10 /pmc/articles/PMC2362909/ /pubmed/10507765 http://dx.doi.org/10.1038/sj.bjc.6690710 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Lin, H-L
Liu, T-Y
Wu, C-W
Chi, C-W
Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title_full Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title_fullStr Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title_full_unstemmed Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title_short Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel
title_sort berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to paclitaxel
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362909/
https://www.ncbi.nlm.nih.gov/pubmed/10507765
http://dx.doi.org/10.1038/sj.bjc.6690710
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