Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization

Retinoids exhibit multiple functions through interaction with nuclear retinoid receptors and have growth-suppressive activity on gastric cancer cells. To better understand the roles of nuclear retinoid receptors during gastric carcinogenesis, we have used in situ hybridization to investigate express...

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Autores principales: Jiang, S-Y, Shen, S-R, Shyu, R-Y, Yu, J-C, Harn, H-J, Yeh, M-Y, Lee, M M-S, Chang, Y-C
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362992/
https://www.ncbi.nlm.nih.gov/pubmed/10389997
http://dx.doi.org/10.1038/sj.bjc.6690340
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author Jiang, S-Y
Shen, S-R
Shyu, R-Y
Yu, J-C
Harn, H-J
Yeh, M-Y
Lee, M M-S
Chang, Y-C
author_facet Jiang, S-Y
Shen, S-R
Shyu, R-Y
Yu, J-C
Harn, H-J
Yeh, M-Y
Lee, M M-S
Chang, Y-C
author_sort Jiang, S-Y
collection PubMed
description Retinoids exhibit multiple functions through interaction with nuclear retinoid receptors and have growth-suppressive activity on gastric cancer cells. To better understand the roles of nuclear retinoid receptors during gastric carcinogenesis, we have used in situ hybridization to investigate expression of retinoic acid receptors (RARs) and retinoid x receptors (RXRs) in premalignant and malignant formalin-fixed paraffin-embedded gastric tissues. Histological sections of eight normal, 17 distal normal and nine gastric cancer tissues were hybridized with non-radioactive RNA probes for subtypes of RAR and RXR. Expression of RARα, RARβ, RARγ, RXRα and RXRβ was found in most cell types in gastric mucosa tissues from normal individuals as well as in distal normal tissues from cancer patients. Expression of RARα and RARβ were found in three and seven cancer tissues, respectively, and levels of RXRα mRNA were significantly decreased in poorly differentiated cancer tissues. Among the five investigated nuclear retinoid receptors, only expression of RARα mRNA was significantly decreased in intestinal metaplasia, dysplasia and cancer tissues when compared to adjacent normal tissues. In conclusion, normal gastric mucosa expressed both RARs and RXRs, which supports the physiological role of retinoic acid on normal gastric mucosa. The decrease in RARα expression in premalignant and malignant gastric tissues suggests a significant role of RARα during gastric carcinogenesis. © 1999 Cancer Research Campaign
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spelling pubmed-23629922009-09-10 Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization Jiang, S-Y Shen, S-R Shyu, R-Y Yu, J-C Harn, H-J Yeh, M-Y Lee, M M-S Chang, Y-C Br J Cancer Regular Article Retinoids exhibit multiple functions through interaction with nuclear retinoid receptors and have growth-suppressive activity on gastric cancer cells. To better understand the roles of nuclear retinoid receptors during gastric carcinogenesis, we have used in situ hybridization to investigate expression of retinoic acid receptors (RARs) and retinoid x receptors (RXRs) in premalignant and malignant formalin-fixed paraffin-embedded gastric tissues. Histological sections of eight normal, 17 distal normal and nine gastric cancer tissues were hybridized with non-radioactive RNA probes for subtypes of RAR and RXR. Expression of RARα, RARβ, RARγ, RXRα and RXRβ was found in most cell types in gastric mucosa tissues from normal individuals as well as in distal normal tissues from cancer patients. Expression of RARα and RARβ were found in three and seven cancer tissues, respectively, and levels of RXRα mRNA were significantly decreased in poorly differentiated cancer tissues. Among the five investigated nuclear retinoid receptors, only expression of RARα mRNA was significantly decreased in intestinal metaplasia, dysplasia and cancer tissues when compared to adjacent normal tissues. In conclusion, normal gastric mucosa expressed both RARs and RXRs, which supports the physiological role of retinoic acid on normal gastric mucosa. The decrease in RARα expression in premalignant and malignant gastric tissues suggests a significant role of RARα during gastric carcinogenesis. © 1999 Cancer Research Campaign Nature Publishing Group 1999-04 /pmc/articles/PMC2362992/ /pubmed/10389997 http://dx.doi.org/10.1038/sj.bjc.6690340 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Jiang, S-Y
Shen, S-R
Shyu, R-Y
Yu, J-C
Harn, H-J
Yeh, M-Y
Lee, M M-S
Chang, Y-C
Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title_full Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title_fullStr Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title_full_unstemmed Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title_short Expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
title_sort expression of nuclear retinoid receptors in normal, premalignant and malignant gastric tissues determined by in situ hybridization
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362992/
https://www.ncbi.nlm.nih.gov/pubmed/10389997
http://dx.doi.org/10.1038/sj.bjc.6690340
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