Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion

MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. In the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobili...

Descripción completa

Detalles Bibliográficos
Autores principales: Závada, J, Závadová, Z, Pastorek, J, Biesová, Z, Jez̆ek, J, Velek, J
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363230/
https://www.ncbi.nlm.nih.gov/pubmed/10839295
http://dx.doi.org/10.1054/bjoc.2000.1111
_version_ 1782153652006289408
author Závada, J
Závadová, Z
Pastorek, J
Biesová, Z
Jez̆ek, J
Velek, J
author_facet Závada, J
Závadová, Z
Pastorek, J
Biesová, Z
Jez̆ek, J
Velek, J
author_sort Závada, J
collection PubMed
description MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. In the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobilized MN/CA IX and that the monoclonal antibody M75 abrogates cell attachment to MN/CA IX. Using synthetic oligopeptides, we identified M75 epitope and located it in the proteoglycan domain, which contains a sixfold tandem repeat of six amino acids GEEDLP. From phage display library of random heptapeptides we identified and chemically synthesized those which compete for the epitope with M75 and inhibit adhesion of cells to MN/CA IX. These heptapeptides might serve as lead compounds for drug design. © 2000 Cancer Research Campaign
format Text
id pubmed-2363230
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23632302009-09-10 Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion Závada, J Závadová, Z Pastorek, J Biesová, Z Jez̆ek, J Velek, J Br J Cancer Regular Article MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. In the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobilized MN/CA IX and that the monoclonal antibody M75 abrogates cell attachment to MN/CA IX. Using synthetic oligopeptides, we identified M75 epitope and located it in the proteoglycan domain, which contains a sixfold tandem repeat of six amino acids GEEDLP. From phage display library of random heptapeptides we identified and chemically synthesized those which compete for the epitope with M75 and inhibit adhesion of cells to MN/CA IX. These heptapeptides might serve as lead compounds for drug design. © 2000 Cancer Research Campaign Nature Publishing Group 2000-06 /pmc/articles/PMC2363230/ /pubmed/10839295 http://dx.doi.org/10.1054/bjoc.2000.1111 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Závada, J
Závadová, Z
Pastorek, J
Biesová, Z
Jez̆ek, J
Velek, J
Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title_full Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title_fullStr Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title_full_unstemmed Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title_short Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion
title_sort human tumour-associated cell adhesion protein mn/ca ix: identification of m75 epitope and of the region mediating cell adhesion
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363230/
https://www.ncbi.nlm.nih.gov/pubmed/10839295
http://dx.doi.org/10.1054/bjoc.2000.1111
work_keys_str_mv AT zavadaj humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion
AT zavadovaz humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion
AT pastorekj humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion
AT biesovaz humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion
AT jezekj humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion
AT velekj humantumourassociatedcelladhesionproteinmncaixidentificationofm75epitopeandoftheregionmediatingcelladhesion

Ejemplares similares