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Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status

HMGI and HMGY are splicing variants of the HMGI(Y) gene and together with HMGI-C, belong to a family of DNA binding proteins involved in maintaining active chromatin conformation and in the regulation of gene transcription. The expression of the HMGI(Y) gene is maximal during embryonic development,...

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Autores principales: Giannini, G, Kim, C J, Marcotullio, L Di, Manfioletti, G, Cardinali, B, Cerignoli, F, Ristori, E, Zani, M, Frati, L, Screpanti, I, Gulino, A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363413/
https://www.ncbi.nlm.nih.gov/pubmed/11076660
http://dx.doi.org/10.1054/bjoc.2000.1494
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author Giannini, G
Kim, C J
Marcotullio, L Di
Manfioletti, G
Cardinali, B
Cerignoli, F
Ristori, E
Zani, M
Frati, L
Screpanti, I
Gulino, A
author_facet Giannini, G
Kim, C J
Marcotullio, L Di
Manfioletti, G
Cardinali, B
Cerignoli, F
Ristori, E
Zani, M
Frati, L
Screpanti, I
Gulino, A
author_sort Giannini, G
collection PubMed
description HMGI and HMGY are splicing variants of the HMGI(Y) gene and together with HMGI-C, belong to a family of DNA binding proteins involved in maintaining active chromatin conformation and in the regulation of gene transcription. The expression of the HMGI(Y) gene is maximal during embryonic development, declines in adult differentiated tissues and is reactivated in most transformed cells in vitro and in many human cancers in vivo. The HMGI(Y) genomic locus is frequently rearranged in mesenchymal tumours, suggesting a biological role for HMGI(Y) gene products in tumour biology. HMGIs are both target and modulators of retinoic acid activity. In fact, HMGI(Y) gene expression is differentially regulated by retinoic acid in retinoid-sensitive and -resistant neuroblastoma cells, while HMGI-C participates in conferring retinoic acid resistance in some neuroblastoma cells. In this paper we show that HMGI and HMGY isoforms are equally regulated by retinoic acid in neuroblastoma cell lines at both RNA and protein levels. More importantly our immunohistochemical analysis shows that, although HMGI(Y) is expressed in all neuroblastic tumours, consistently higher levels are observed in less differentiated neuroblastomas compared to more differentiated ganglioneuromas, indicating that HMGI(Y) expression should be evaluated as a potential diagnostic and prognostic marker in neuroblastic tumours. © 2000 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23634132009-09-10 Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status Giannini, G Kim, C J Marcotullio, L Di Manfioletti, G Cardinali, B Cerignoli, F Ristori, E Zani, M Frati, L Screpanti, I Gulino, A Br J Cancer Regular Article HMGI and HMGY are splicing variants of the HMGI(Y) gene and together with HMGI-C, belong to a family of DNA binding proteins involved in maintaining active chromatin conformation and in the regulation of gene transcription. The expression of the HMGI(Y) gene is maximal during embryonic development, declines in adult differentiated tissues and is reactivated in most transformed cells in vitro and in many human cancers in vivo. The HMGI(Y) genomic locus is frequently rearranged in mesenchymal tumours, suggesting a biological role for HMGI(Y) gene products in tumour biology. HMGIs are both target and modulators of retinoic acid activity. In fact, HMGI(Y) gene expression is differentially regulated by retinoic acid in retinoid-sensitive and -resistant neuroblastoma cells, while HMGI-C participates in conferring retinoic acid resistance in some neuroblastoma cells. In this paper we show that HMGI and HMGY isoforms are equally regulated by retinoic acid in neuroblastoma cell lines at both RNA and protein levels. More importantly our immunohistochemical analysis shows that, although HMGI(Y) is expressed in all neuroblastic tumours, consistently higher levels are observed in less differentiated neuroblastomas compared to more differentiated ganglioneuromas, indicating that HMGI(Y) expression should be evaluated as a potential diagnostic and prognostic marker in neuroblastic tumours. © 2000 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2000-12 2000-11-22 /pmc/articles/PMC2363413/ /pubmed/11076660 http://dx.doi.org/10.1054/bjoc.2000.1494 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Giannini, G
Kim, C J
Marcotullio, L Di
Manfioletti, G
Cardinali, B
Cerignoli, F
Ristori, E
Zani, M
Frati, L
Screpanti, I
Gulino, A
Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title_full Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title_fullStr Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title_full_unstemmed Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title_short Expression of the HMGI(Y) gene products in human neuroblastic tumours correlates with differentiation status
title_sort expression of the hmgi(y) gene products in human neuroblastic tumours correlates with differentiation status
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363413/
https://www.ncbi.nlm.nih.gov/pubmed/11076660
http://dx.doi.org/10.1054/bjoc.2000.1494
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