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HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium
HERG K(+)channels, besides contributing to regulate cardiac and neuronal excitability, are preferentially expressed in tumour cell lines of different histogenesis, where their role in the development and maintenance of the neoplastic phenotype is under study. We show here that both herg gene and HER...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2000
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363441/ https://www.ncbi.nlm.nih.gov/pubmed/11104572 http://dx.doi.org/10.1054/bjoc.2000.1497 |
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author | Cherubini, A Taddei, G L Crociani, O Paglierani, M Buccoliero, A M Fontana, L Noci, I Borri, P Borrani, E Giachi, M Becchetti, A Rosati, B Wanke, E Olivotto, M Arcangeli, A |
author_facet | Cherubini, A Taddei, G L Crociani, O Paglierani, M Buccoliero, A M Fontana, L Noci, I Borri, P Borrani, E Giachi, M Becchetti, A Rosati, B Wanke, E Olivotto, M Arcangeli, A |
author_sort | Cherubini, A |
collection | PubMed |
description | HERG K(+)channels, besides contributing to regulate cardiac and neuronal excitability, are preferentially expressed in tumour cell lines of different histogenesis, where their role in the development and maintenance of the neoplastic phenotype is under study. We show here that both herg gene and HERG protein are expressed with high frequency in primary human endometrial cancers, as compared to normal and hyperplastic endometrium. RT-PCR and immunohistochemistry, using specific anti-HERG antibodies developed in our laboratory, were applied to tissue specimens obtained from 18 endometrial cancers and 11 non-cancerous endometrial tissues. herg RNA and HERG protein are expressed in 67% and 82%, respectively, of cancerous, while in only 18% of non-cancerous tissues. In particular, no expression was found in endometrial hyperplasia. Moreover, electrophysiological experiments confirmed the presence of functioning HERG channels on the plasma membrane of tumour cells. On the whole, these data are the first demonstration of the presence of HERG channels in primary human neoplasias, and could candidate HERG as a potential tool capable of marking cancerous versus hyperplastic endometrial growth. © 2000 Cancer Research Campaign http://www.bjcancer.com |
format | Text |
id | pubmed-2363441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23634412009-09-10 HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium Cherubini, A Taddei, G L Crociani, O Paglierani, M Buccoliero, A M Fontana, L Noci, I Borri, P Borrani, E Giachi, M Becchetti, A Rosati, B Wanke, E Olivotto, M Arcangeli, A Br J Cancer Regular Article HERG K(+)channels, besides contributing to regulate cardiac and neuronal excitability, are preferentially expressed in tumour cell lines of different histogenesis, where their role in the development and maintenance of the neoplastic phenotype is under study. We show here that both herg gene and HERG protein are expressed with high frequency in primary human endometrial cancers, as compared to normal and hyperplastic endometrium. RT-PCR and immunohistochemistry, using specific anti-HERG antibodies developed in our laboratory, were applied to tissue specimens obtained from 18 endometrial cancers and 11 non-cancerous endometrial tissues. herg RNA and HERG protein are expressed in 67% and 82%, respectively, of cancerous, while in only 18% of non-cancerous tissues. In particular, no expression was found in endometrial hyperplasia. Moreover, electrophysiological experiments confirmed the presence of functioning HERG channels on the plasma membrane of tumour cells. On the whole, these data are the first demonstration of the presence of HERG channels in primary human neoplasias, and could candidate HERG as a potential tool capable of marking cancerous versus hyperplastic endometrial growth. © 2000 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2000-12 /pmc/articles/PMC2363441/ /pubmed/11104572 http://dx.doi.org/10.1054/bjoc.2000.1497 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Cherubini, A Taddei, G L Crociani, O Paglierani, M Buccoliero, A M Fontana, L Noci, I Borri, P Borrani, E Giachi, M Becchetti, A Rosati, B Wanke, E Olivotto, M Arcangeli, A HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title | HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title_full | HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title_fullStr | HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title_full_unstemmed | HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title_short | HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
title_sort | herg potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363441/ https://www.ncbi.nlm.nih.gov/pubmed/11104572 http://dx.doi.org/10.1054/bjoc.2000.1497 |
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