Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes

The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma. The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of wh...

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Autores principales: Calogero, A, Timmer-Bosscha, H, Koops, H Schraffordt, Tiebosch, A T M G, Mulder, N H, Hospers, G A P
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363472/
https://www.ncbi.nlm.nih.gov/pubmed/10901368
http://dx.doi.org/10.1054/bjoc.2000.1282
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author Calogero, A
Timmer-Bosscha, H
Koops, H Schraffordt
Tiebosch, A T M G
Mulder, N H
Hospers, G A P
author_facet Calogero, A
Timmer-Bosscha, H
Koops, H Schraffordt
Tiebosch, A T M G
Mulder, N H
Hospers, G A P
author_sort Calogero, A
collection PubMed
description The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma. The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of which one patient was treated with granulocyte-colony stimulating factor. Lymph nodes of nine patients without malignant melanoma were tested and four cell lines of various other tumours. Six of the nine non-melanoma lymph nodes were positive in this assay. The sensitivity was tested in a spike experiment in vitro, using a melanoma cell line. The detection limit was ten melanoma cells per 10(7)peripheral blood lymphocytes. © 2000 Cancer Research Campaign
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spelling pubmed-23634722009-09-10 Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes Calogero, A Timmer-Bosscha, H Koops, H Schraffordt Tiebosch, A T M G Mulder, N H Hospers, G A P Br J Cancer Regular Article The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma. The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of which one patient was treated with granulocyte-colony stimulating factor. Lymph nodes of nine patients without malignant melanoma were tested and four cell lines of various other tumours. Six of the nine non-melanoma lymph nodes were positive in this assay. The sensitivity was tested in a spike experiment in vitro, using a melanoma cell line. The detection limit was ten melanoma cells per 10(7)peripheral blood lymphocytes. © 2000 Cancer Research Campaign Nature Publishing Group 2000-07 2000-06-15 /pmc/articles/PMC2363472/ /pubmed/10901368 http://dx.doi.org/10.1054/bjoc.2000.1282 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Calogero, A
Timmer-Bosscha, H
Koops, H Schraffordt
Tiebosch, A T M G
Mulder, N H
Hospers, G A P
Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title_full Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title_fullStr Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title_full_unstemmed Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title_short Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
title_sort limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363472/
https://www.ncbi.nlm.nih.gov/pubmed/10901368
http://dx.doi.org/10.1054/bjoc.2000.1282
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