Cargando…

Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer

Four different genes were identified by immunoscreening of a cDNA expression library from the human prostate cancer cell line DU145 with allogeneic sera from four prostate cancer patients. A cDNA encoding the nucleolar protein No55 was further analysed and shown to be expressed at the mRNA level in...

Descripción completa

Detalles Bibliográficos
Autores principales: Fosså, A, Siebert, R, Aasheim, H-C, Mælandsmo, G M, Berner, A, Fosså, S D, Paus, E, Smeland, E B, Gaudernack, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363543/
https://www.ncbi.nlm.nih.gov/pubmed/10952778
http://dx.doi.org/10.1054/bjoc.2000.1365
_version_ 1782153730839281664
author Fosså, A
Siebert, R
Aasheim, H-C
Mælandsmo, G M
Berner, A
Fosså, S D
Paus, E
Smeland, E B
Gaudernack, G
author_facet Fosså, A
Siebert, R
Aasheim, H-C
Mælandsmo, G M
Berner, A
Fosså, S D
Paus, E
Smeland, E B
Gaudernack, G
author_sort Fosså, A
collection PubMed
description Four different genes were identified by immunoscreening of a cDNA expression library from the human prostate cancer cell line DU145 with allogeneic sera from four prostate cancer patients. A cDNA encoding the nucleolar protein No55 was further analysed and shown to be expressed at the mRNA level in several normal tissues, including ovaries, pancreas and prostate and in human prostate cancer cell lines PC-3, PC-3m and LNCaP. By reverse transcriptase/polymerase chain reaction, expression of No55 was several-fold higher in two out of nine prostate cancer primary tumours and two out of two metastatic lesions, compared to normal prostate tissue. Antibodies to No55 were detected in sera from seven out of 47 prostate cancer patients but not in sera from 20 healthy male controls. Sequence analysis of the No55 open reading frame from normal and tumour tissues revealed no tumour-specific mutations. The No55 gene was located to chromosome 17q21, a region reported to be partially deleted in prostate cancer. Considering the immunogenicity of the No55 protein in the tumour host, the expression profile and chromosomal localization of the corresponding gene, studies evaluating No55 as a potential antigen for immunological studies in prostate cancer may be warranted. © 2000 Cancer Research Campaign
format Text
id pubmed-2363543
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23635432009-09-10 Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer Fosså, A Siebert, R Aasheim, H-C Mælandsmo, G M Berner, A Fosså, S D Paus, E Smeland, E B Gaudernack, G Br J Cancer Regular Article Four different genes were identified by immunoscreening of a cDNA expression library from the human prostate cancer cell line DU145 with allogeneic sera from four prostate cancer patients. A cDNA encoding the nucleolar protein No55 was further analysed and shown to be expressed at the mRNA level in several normal tissues, including ovaries, pancreas and prostate and in human prostate cancer cell lines PC-3, PC-3m and LNCaP. By reverse transcriptase/polymerase chain reaction, expression of No55 was several-fold higher in two out of nine prostate cancer primary tumours and two out of two metastatic lesions, compared to normal prostate tissue. Antibodies to No55 were detected in sera from seven out of 47 prostate cancer patients but not in sera from 20 healthy male controls. Sequence analysis of the No55 open reading frame from normal and tumour tissues revealed no tumour-specific mutations. The No55 gene was located to chromosome 17q21, a region reported to be partially deleted in prostate cancer. Considering the immunogenicity of the No55 protein in the tumour host, the expression profile and chromosomal localization of the corresponding gene, studies evaluating No55 as a potential antigen for immunological studies in prostate cancer may be warranted. © 2000 Cancer Research Campaign Nature Publishing Group 2000-09 2000-08-17 /pmc/articles/PMC2363543/ /pubmed/10952778 http://dx.doi.org/10.1054/bjoc.2000.1365 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Fosså, A
Siebert, R
Aasheim, H-C
Mælandsmo, G M
Berner, A
Fosså, S D
Paus, E
Smeland, E B
Gaudernack, G
Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title_full Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title_fullStr Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title_full_unstemmed Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title_short Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer
title_sort identification of nucleolar protein no55 as a tumour-associated autoantigen in patients with prostate cancer
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363543/
https://www.ncbi.nlm.nih.gov/pubmed/10952778
http://dx.doi.org/10.1054/bjoc.2000.1365
work_keys_str_mv AT fossaa identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT siebertr identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT aasheimhc identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT mælandsmogm identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT bernera identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT fossasd identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT pause identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT smelandeb identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer
AT gaudernackg identificationofnucleolarproteinno55asatumourassociatedautoantigeninpatientswithprostatecancer