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No SMAD4 hypermethylation in colorectal cancer

The chromosome region 18q21 is frequently deleted in colorectal cancers. Three candidate tumour suppressor genes, DCC, SMAD4 and SMAD2, map to this region. The SMAD4(DPC4) gene was recently identified as a candidate pancreatic cancer suppressor gene. It is also a gene for juvenile polyposis tumour p...

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Autores principales: Roth, S, Laiho, P, Salovaara, R, Launonen, V, Aaltonen, L A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363563/
https://www.ncbi.nlm.nih.gov/pubmed/10993648
http://dx.doi.org/10.1054/bjoc.2000.1387
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author Roth, S
Laiho, P
Salovaara, R
Launonen, V
Aaltonen, L A
author_facet Roth, S
Laiho, P
Salovaara, R
Launonen, V
Aaltonen, L A
author_sort Roth, S
collection PubMed
description The chromosome region 18q21 is frequently deleted in colorectal cancers. Three candidate tumour suppressor genes, DCC, SMAD4 and SMAD2, map to this region. The SMAD4(DPC4) gene was recently identified as a candidate pancreatic cancer suppressor gene. It is also a gene for juvenile polyposis tumour predisposition syndrome. Somatic SMAD4 mutations have been detected in some colorectal carcinomas. However, the frequency of these mutations is relatively low, and whether SMAD4 plays a key role in colorectal tumorigenesis is still unclear. In addition to loss of chromosomal material and intragenic mutations there is a third mechanism, DNA methylation, which may have an important role in gene inactivation. In the present study, we examined whether promoter hypermethylation could be a mechanism for SMAD4 inactivation. In total, 42 colorectal tumours were selected for the methylation analysis and no evidence of promoter hypermethylation was found. Our result suggests that hypermethylation of the SMAD4 promoter region is not a frequent event in colorectal tumorigenesis. © 2000 Cancer Research Campaign
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spelling pubmed-23635632009-09-10 No SMAD4 hypermethylation in colorectal cancer Roth, S Laiho, P Salovaara, R Launonen, V Aaltonen, L A Br J Cancer Regular Article The chromosome region 18q21 is frequently deleted in colorectal cancers. Three candidate tumour suppressor genes, DCC, SMAD4 and SMAD2, map to this region. The SMAD4(DPC4) gene was recently identified as a candidate pancreatic cancer suppressor gene. It is also a gene for juvenile polyposis tumour predisposition syndrome. Somatic SMAD4 mutations have been detected in some colorectal carcinomas. However, the frequency of these mutations is relatively low, and whether SMAD4 plays a key role in colorectal tumorigenesis is still unclear. In addition to loss of chromosomal material and intragenic mutations there is a third mechanism, DNA methylation, which may have an important role in gene inactivation. In the present study, we examined whether promoter hypermethylation could be a mechanism for SMAD4 inactivation. In total, 42 colorectal tumours were selected for the methylation analysis and no evidence of promoter hypermethylation was found. Our result suggests that hypermethylation of the SMAD4 promoter region is not a frequent event in colorectal tumorigenesis. © 2000 Cancer Research Campaign Nature Publishing Group 2000-10 /pmc/articles/PMC2363563/ /pubmed/10993648 http://dx.doi.org/10.1054/bjoc.2000.1387 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Roth, S
Laiho, P
Salovaara, R
Launonen, V
Aaltonen, L A
No SMAD4 hypermethylation in colorectal cancer
title No SMAD4 hypermethylation in colorectal cancer
title_full No SMAD4 hypermethylation in colorectal cancer
title_fullStr No SMAD4 hypermethylation in colorectal cancer
title_full_unstemmed No SMAD4 hypermethylation in colorectal cancer
title_short No SMAD4 hypermethylation in colorectal cancer
title_sort no smad4 hypermethylation in colorectal cancer
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363563/
https://www.ncbi.nlm.nih.gov/pubmed/10993648
http://dx.doi.org/10.1054/bjoc.2000.1387
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