Cargando…

CD55 is over-expressed in the tumour environment

CD55 is a protein that protects cells from complement-mediated attack. 791Tgp72 is an antigen which has been used succesfully as a target for both tumour imaging and cancer vaccines. 791Tgp72 has recently been identified as CD55. Quantitative expression of CD55 in the tumour environment was therefor...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, L, Spendlove, I, Morgan, J, Durrant, L G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363604/
https://www.ncbi.nlm.nih.gov/pubmed/11139317
http://dx.doi.org/10.1054/bjoc.2000.1570
_version_ 1782153746164219904
author Li, L
Spendlove, I
Morgan, J
Durrant, L G
author_facet Li, L
Spendlove, I
Morgan, J
Durrant, L G
author_sort Li, L
collection PubMed
description CD55 is a protein that protects cells from complement-mediated attack. 791Tgp72 is an antigen which has been used succesfully as a target for both tumour imaging and cancer vaccines. 791Tgp72 has recently been identified as CD55. Quantitative expression of CD55 in the tumour environment was therefore studied. Tumour cells showed a 4–100-fold increase in CD55 cell surface expression when compared to normal cells. Immunohistochemical staining of colorectal tumours also revealed high expression of CD55 in the stroma. To examine the source of this stromal CD55 the ability of both epithelial cells and endothelial cells to produce extracellular CD55 was measured. Tumour cell lines deposit CD55 into their extracellular matrix (ECM) in direct proportion to their cell surface expression. In contrast the ECM from HUVEC cells contained large amounts of CD55 despite expressing low levels of CD55 on their cell surface. Furthermore expression of CD55 on HUVEC cells was increased by exposure to VEGF. Although it remains unclear why CD55 is upregulated in the tumour environment its high level of expression on tumour cells and associated endothelium may explain why it is a good target for both imaging and immunotherapy. © 2001 Cancer Research Campaign http://www.bjcancer.com
format Text
id pubmed-2363604
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23636042009-09-10 CD55 is over-expressed in the tumour environment Li, L Spendlove, I Morgan, J Durrant, L G Br J Cancer Regular Article CD55 is a protein that protects cells from complement-mediated attack. 791Tgp72 is an antigen which has been used succesfully as a target for both tumour imaging and cancer vaccines. 791Tgp72 has recently been identified as CD55. Quantitative expression of CD55 in the tumour environment was therefore studied. Tumour cells showed a 4–100-fold increase in CD55 cell surface expression when compared to normal cells. Immunohistochemical staining of colorectal tumours also revealed high expression of CD55 in the stroma. To examine the source of this stromal CD55 the ability of both epithelial cells and endothelial cells to produce extracellular CD55 was measured. Tumour cell lines deposit CD55 into their extracellular matrix (ECM) in direct proportion to their cell surface expression. In contrast the ECM from HUVEC cells contained large amounts of CD55 despite expressing low levels of CD55 on their cell surface. Furthermore expression of CD55 on HUVEC cells was increased by exposure to VEGF. Although it remains unclear why CD55 is upregulated in the tumour environment its high level of expression on tumour cells and associated endothelium may explain why it is a good target for both imaging and immunotherapy. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-01 2001-01-01 /pmc/articles/PMC2363604/ /pubmed/11139317 http://dx.doi.org/10.1054/bjoc.2000.1570 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Li, L
Spendlove, I
Morgan, J
Durrant, L G
CD55 is over-expressed in the tumour environment
title CD55 is over-expressed in the tumour environment
title_full CD55 is over-expressed in the tumour environment
title_fullStr CD55 is over-expressed in the tumour environment
title_full_unstemmed CD55 is over-expressed in the tumour environment
title_short CD55 is over-expressed in the tumour environment
title_sort cd55 is over-expressed in the tumour environment
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363604/
https://www.ncbi.nlm.nih.gov/pubmed/11139317
http://dx.doi.org/10.1054/bjoc.2000.1570
work_keys_str_mv AT lil cd55isoverexpressedinthetumourenvironment
AT spendlovei cd55isoverexpressedinthetumourenvironment
AT morganj cd55isoverexpressedinthetumourenvironment
AT durrantlg cd55isoverexpressedinthetumourenvironment