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Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic di...

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Autores principales: Ladner, D P, Steiner, R A, Allemann, J, Haller, U, Walt, H
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363621/
https://www.ncbi.nlm.nih.gov/pubmed/11139309
http://dx.doi.org/10.1054/bjoc.2000.1532
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author Ladner, D P
Steiner, R A
Allemann, J
Haller, U
Walt, H
author_facet Ladner, D P
Steiner, R A
Allemann, J
Haller, U
Walt, H
author_sort Ladner, D P
collection PubMed
description Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic diagnosis of human primary mammary tumours. The study included 16 patients with palpable breast tumours. Aminolevulinic acid was administered at a concentration of 40 mg kg(−1)bodyweight 150–420 min prior to tumourectomy. Intraoperatively blue light (405 nm) was applied to the operation site. Sections of the excised tumour and some lymph nodes were prepared and analysed with a fluorescent microscope. All primary mammary tumour tissues showed significantly higher fluorescence intensity than surrounding normal mammary tissue. Fluorescence of the mammary tumours could also be discriminated macroscopically and intraoperatively. Fluorescence intensity in nonmetastatic lymph node tissue was higher in 2 out of 3 patients than in primary tumour tissue. By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients. We suggest that photodynamic diagnosis with aminolevulinic acid for breast tumours should be further investigated and developed for intraoperative use and may well be a simple tool for better intraoperative diagnosis and recognition of tumour margins. We hypothesize that lymph node metastasis of breast tumours will not be detectable by this method. © 2001 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23636212009-09-10 Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid Ladner, D P Steiner, R A Allemann, J Haller, U Walt, H Br J Cancer Regular Article Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic diagnosis of human primary mammary tumours. The study included 16 patients with palpable breast tumours. Aminolevulinic acid was administered at a concentration of 40 mg kg(−1)bodyweight 150–420 min prior to tumourectomy. Intraoperatively blue light (405 nm) was applied to the operation site. Sections of the excised tumour and some lymph nodes were prepared and analysed with a fluorescent microscope. All primary mammary tumour tissues showed significantly higher fluorescence intensity than surrounding normal mammary tissue. Fluorescence of the mammary tumours could also be discriminated macroscopically and intraoperatively. Fluorescence intensity in nonmetastatic lymph node tissue was higher in 2 out of 3 patients than in primary tumour tissue. By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients. We suggest that photodynamic diagnosis with aminolevulinic acid for breast tumours should be further investigated and developed for intraoperative use and may well be a simple tool for better intraoperative diagnosis and recognition of tumour margins. We hypothesize that lymph node metastasis of breast tumours will not be detectable by this method. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-01 2001-01-01 /pmc/articles/PMC2363621/ /pubmed/11139309 http://dx.doi.org/10.1054/bjoc.2000.1532 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Ladner, D P
Steiner, R A
Allemann, J
Haller, U
Walt, H
Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title_full Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title_fullStr Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title_full_unstemmed Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title_short Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
title_sort photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363621/
https://www.ncbi.nlm.nih.gov/pubmed/11139309
http://dx.doi.org/10.1054/bjoc.2000.1532
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