Cargando…

Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells

We report that all- trans retinoic acid (ATRA) enhanced the toxicity of docetaxel against DU145 and LNCaP prostate cancer cells, and that the nature of the interaction between ATRA and docetaxel was highly synergistic. Docetaxel-induced apoptotic cell death was associated with phosphorylation and he...

Descripción completa

Detalles Bibliográficos
Autores principales: Nehmé, A, Varadarajan, P, Sellakumar, G, Gerhold, M, Niedner, H, Zhang, Q, Lin, X, Christen, R D
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363662/
https://www.ncbi.nlm.nih.gov/pubmed/11384110
http://dx.doi.org/10.1054/bjoc.2001.1818
_version_ 1782153760761446400
author Nehmé, A
Varadarajan, P
Sellakumar, G
Gerhold, M
Niedner, H
Zhang, Q
Lin, X
Christen, R D
author_facet Nehmé, A
Varadarajan, P
Sellakumar, G
Gerhold, M
Niedner, H
Zhang, Q
Lin, X
Christen, R D
author_sort Nehmé, A
collection PubMed
description We report that all- trans retinoic acid (ATRA) enhanced the toxicity of docetaxel against DU145 and LNCaP prostate cancer cells, and that the nature of the interaction between ATRA and docetaxel was highly synergistic. Docetaxel-induced apoptotic cell death was associated with phosphorylation and hence inactivation of Bcl-2. ATRA enhanced docetaxel-induced apoptosis and combined treatment with ATRA and docetaxel resulted in down-regulation of Bcl-2. Docetaxel caused phosphorylation and hence inactivation of cdc2 kinase result ing in G2/M arrest. ATRA inhibited docetaxel-induced phosphorylation of cdc2 resulting in activation of cdc2 kinase and partial reversal of the G2/M arrest. ATRA also inhibited docetaxel-induced activation of MAPK indicating that the effects of docetaxel and ATRA on cdc2 phosphorylation are dependent on MAPK. We conclude that ATRA synergistically enhances docetaxel toxicity by down-regulating Bcl-2 expression and partially reverses the docetaxel-induced G2/M arrest by inhibiting docetaxel-induced cdc2 phosphorylation in a pathway that is dependent on MAPK. © 2001 Cancer Research Campaign http://www.bjcancer.com
format Text
id pubmed-2363662
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23636622009-09-10 Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells Nehmé, A Varadarajan, P Sellakumar, G Gerhold, M Niedner, H Zhang, Q Lin, X Christen, R D Br J Cancer Regular Article We report that all- trans retinoic acid (ATRA) enhanced the toxicity of docetaxel against DU145 and LNCaP prostate cancer cells, and that the nature of the interaction between ATRA and docetaxel was highly synergistic. Docetaxel-induced apoptotic cell death was associated with phosphorylation and hence inactivation of Bcl-2. ATRA enhanced docetaxel-induced apoptosis and combined treatment with ATRA and docetaxel resulted in down-regulation of Bcl-2. Docetaxel caused phosphorylation and hence inactivation of cdc2 kinase result ing in G2/M arrest. ATRA inhibited docetaxel-induced phosphorylation of cdc2 resulting in activation of cdc2 kinase and partial reversal of the G2/M arrest. ATRA also inhibited docetaxel-induced activation of MAPK indicating that the effects of docetaxel and ATRA on cdc2 phosphorylation are dependent on MAPK. We conclude that ATRA synergistically enhances docetaxel toxicity by down-regulating Bcl-2 expression and partially reverses the docetaxel-induced G2/M arrest by inhibiting docetaxel-induced cdc2 phosphorylation in a pathway that is dependent on MAPK. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-06 /pmc/articles/PMC2363662/ /pubmed/11384110 http://dx.doi.org/10.1054/bjoc.2001.1818 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Nehmé, A
Varadarajan, P
Sellakumar, G
Gerhold, M
Niedner, H
Zhang, Q
Lin, X
Christen, R D
Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title_full Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title_fullStr Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title_full_unstemmed Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title_short Modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
title_sort modulation of docetaxel-induced apoptosis and cell cycle arrest by all- trans retinoic acid in prostate cancer cells
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363662/
https://www.ncbi.nlm.nih.gov/pubmed/11384110
http://dx.doi.org/10.1054/bjoc.2001.1818
work_keys_str_mv AT nehmea modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT varadarajanp modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT sellakumarg modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT gerholdm modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT niednerh modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT zhangq modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT linx modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells
AT christenrd modulationofdocetaxelinducedapoptosisandcellcyclearrestbyalltransretinoicacidinprostatecancercells