Detection of circulating carcinoma cells by telomerase activity

Telomerase has been shown to be a marker of epithelial cancer cells. We developed a method that allows the detection of circulating carcinoma cells in the blood of cancer patients. Circulating epithelial cells are harvested from peripheral blood mononuclear cells by immunomagnetic separation using B...

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Autores principales: Gauthier, L R, Granotier, C, Soria, J-C, Faivre, S, Boige, V, Raymond, E, Boussin, F D
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363781/
https://www.ncbi.nlm.nih.gov/pubmed/11237383
http://dx.doi.org/10.1054/bjoc.2000.1662
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author Gauthier, L R
Granotier, C
Soria, J-C
Faivre, S
Boige, V
Raymond, E
Boussin, F D
author_facet Gauthier, L R
Granotier, C
Soria, J-C
Faivre, S
Boige, V
Raymond, E
Boussin, F D
author_sort Gauthier, L R
collection PubMed
description Telomerase has been shown to be a marker of epithelial cancer cells. We developed a method that allows the detection of circulating carcinoma cells in the blood of cancer patients. Circulating epithelial cells are harvested from peripheral blood mononuclear cells by immunomagnetic separation using BerEP4-coated beads. A telomeric repeat amplification protocol (TRAP)-ELISA is then used to measure telomerase in harvested epithelial cells. This method is specific and sensitive as demonstrated by experiments using BerEP4-positive and negative cell lines. Whereas we never found telomerase activity in harvested epithelial cells (HEC) samples from 30/30 healthy donors, we have detected telomerase activity in HEC from 11/15 (73%) patients with stage IIIB or IV non-small cell lung cancer (NSCLC) patients and from 8/11 (72%) stage C or D (Dukes classification) colon cancer patients. This non-invasive method could be of great value as a diagnostic or prognostic marker, or for monitoring cancer progression. © 2001 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23637812009-09-10 Detection of circulating carcinoma cells by telomerase activity Gauthier, L R Granotier, C Soria, J-C Faivre, S Boige, V Raymond, E Boussin, F D Br J Cancer Regular Article Telomerase has been shown to be a marker of epithelial cancer cells. We developed a method that allows the detection of circulating carcinoma cells in the blood of cancer patients. Circulating epithelial cells are harvested from peripheral blood mononuclear cells by immunomagnetic separation using BerEP4-coated beads. A telomeric repeat amplification protocol (TRAP)-ELISA is then used to measure telomerase in harvested epithelial cells. This method is specific and sensitive as demonstrated by experiments using BerEP4-positive and negative cell lines. Whereas we never found telomerase activity in harvested epithelial cells (HEC) samples from 30/30 healthy donors, we have detected telomerase activity in HEC from 11/15 (73%) patients with stage IIIB or IV non-small cell lung cancer (NSCLC) patients and from 8/11 (72%) stage C or D (Dukes classification) colon cancer patients. This non-invasive method could be of great value as a diagnostic or prognostic marker, or for monitoring cancer progression. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-03 /pmc/articles/PMC2363781/ /pubmed/11237383 http://dx.doi.org/10.1054/bjoc.2000.1662 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Gauthier, L R
Granotier, C
Soria, J-C
Faivre, S
Boige, V
Raymond, E
Boussin, F D
Detection of circulating carcinoma cells by telomerase activity
title Detection of circulating carcinoma cells by telomerase activity
title_full Detection of circulating carcinoma cells by telomerase activity
title_fullStr Detection of circulating carcinoma cells by telomerase activity
title_full_unstemmed Detection of circulating carcinoma cells by telomerase activity
title_short Detection of circulating carcinoma cells by telomerase activity
title_sort detection of circulating carcinoma cells by telomerase activity
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363781/
https://www.ncbi.nlm.nih.gov/pubmed/11237383
http://dx.doi.org/10.1054/bjoc.2000.1662
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