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Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts
Bystander responses underlie some of the current efforts to develop gene therapy approaches for cancer treatment. Similarly, they may have a role in strategies to treat tumours with targeted radioisotopes. In this study we show direct evidence for the production of a radiation-induced bystander resp...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2001
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363796/ https://www.ncbi.nlm.nih.gov/pubmed/11237389 http://dx.doi.org/10.1054/bjoc.2000.1665 |
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author | Belyakov, O V Malcolmson, A M Folkard, M Prise, K M Michael, B D |
author_facet | Belyakov, O V Malcolmson, A M Folkard, M Prise, K M Michael, B D |
author_sort | Belyakov, O V |
collection | PubMed |
description | Bystander responses underlie some of the current efforts to develop gene therapy approaches for cancer treatment. Similarly, they may have a role in strategies to treat tumours with targeted radioisotopes. In this study we show direct evidence for the production of a radiation-induced bystander response in primary human fibroblasts. We utilize a novel approach of using a charged-particle microbeam, which allows individual cells within a population to be selected and targeted with counted charged particles. Individual primary human fibroblasts within a population of 600–800 cells were targeted with between 1 and 15 helium ions (effectively, α-particles). The charged particles were delivered through the centre of the nucleus with an accuracy of ± 2 μm and a detection and counting efficiency of greater than 99%. When scored 3 days later, even though only a single cell had been targeted, typically an additional 80–100 damaged cells were observed in the surviving population of about 5000 cells. The yield of damaged cells was independent of the number of charged particles delivered to the targeted cell. Similar results of a 2–3-fold increase in the background level of damage present in the population were observed whether 1 or 4 cells were targeted within the dish. Also, when 200 cells within one quadrant of the dish were exposed to radiation, there was a 2–3-fold increase in the damage level in an unexposed quadrant of the dish. This effect was independent of the presence of serum in the culture medium and was only observed when a cell was targeted, but not when only the medium was exposed, confirming that a cell-mediated response is involved. © 2001 Cancer Research Campaign http://www.bjcancer.com |
format | Text |
id | pubmed-2363796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23637962009-09-10 Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts Belyakov, O V Malcolmson, A M Folkard, M Prise, K M Michael, B D Br J Cancer Regular Article Bystander responses underlie some of the current efforts to develop gene therapy approaches for cancer treatment. Similarly, they may have a role in strategies to treat tumours with targeted radioisotopes. In this study we show direct evidence for the production of a radiation-induced bystander response in primary human fibroblasts. We utilize a novel approach of using a charged-particle microbeam, which allows individual cells within a population to be selected and targeted with counted charged particles. Individual primary human fibroblasts within a population of 600–800 cells were targeted with between 1 and 15 helium ions (effectively, α-particles). The charged particles were delivered through the centre of the nucleus with an accuracy of ± 2 μm and a detection and counting efficiency of greater than 99%. When scored 3 days later, even though only a single cell had been targeted, typically an additional 80–100 damaged cells were observed in the surviving population of about 5000 cells. The yield of damaged cells was independent of the number of charged particles delivered to the targeted cell. Similar results of a 2–3-fold increase in the background level of damage present in the population were observed whether 1 or 4 cells were targeted within the dish. Also, when 200 cells within one quadrant of the dish were exposed to radiation, there was a 2–3-fold increase in the damage level in an unexposed quadrant of the dish. This effect was independent of the presence of serum in the culture medium and was only observed when a cell was targeted, but not when only the medium was exposed, confirming that a cell-mediated response is involved. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-03 /pmc/articles/PMC2363796/ /pubmed/11237389 http://dx.doi.org/10.1054/bjoc.2000.1665 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Belyakov, O V Malcolmson, A M Folkard, M Prise, K M Michael, B D Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title | Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title_full | Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title_fullStr | Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title_full_unstemmed | Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title_short | Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
title_sort | direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363796/ https://www.ncbi.nlm.nih.gov/pubmed/11237389 http://dx.doi.org/10.1054/bjoc.2000.1665 |
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