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Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF

Increased expression of VEGF in several types of tumours has been shown to correlate with poor prognosis. We used a replication-deficient adenoviral vector containing antisense VEGF cDNA (Ad5CMV-αVEGF) to down-regulate VEGF expression and increase the efficiency of delivery of the antisense sequence...

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Autores principales: Im, S-A, Kim, J-S, Gomez-Manzano, C, Fueyo, J, Liu, T-J, Cho, M-S, Seong, C-M, Lee, S N, Hong, Y-K, Yung, W K A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363890/
https://www.ncbi.nlm.nih.gov/pubmed/11336478
http://dx.doi.org/10.1054/bjoc.2000.1734
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author Im, S-A
Kim, J-S
Gomez-Manzano, C
Fueyo, J
Liu, T-J
Cho, M-S
Seong, C-M
Lee, S N
Hong, Y-K
Yung, W K A
author_facet Im, S-A
Kim, J-S
Gomez-Manzano, C
Fueyo, J
Liu, T-J
Cho, M-S
Seong, C-M
Lee, S N
Hong, Y-K
Yung, W K A
author_sort Im, S-A
collection PubMed
description Increased expression of VEGF in several types of tumours has been shown to correlate with poor prognosis. We used a replication-deficient adenoviral vector containing antisense VEGF cDNA (Ad5CMV-αVEGF) to down-regulate VEGF expression and increase the efficiency of delivery of the antisense sequence in the human breast cancer cell line MDA231-MB. Transfection of these cells with Ad5CMV-αVEGF in vitro reduced secreted levels of VEGF protein without affecting cell growth. Moreover, injection of the Ad5CMV-αVEGF vector into intramammary xenografts of these cells established in nude mice inhibited tumour growth and reduced the amount of VEGF protein and the density of microvessels in those tumours relative to tumours treated with the control vector Ad5(dl312). Our results showed that antisense VEGF (165) cDNA was efficiently delivered in vivo via an adenoviral vector and that this treatment significantly inhibited the growth of established experimental breast tumours. The Ad5CMV-αVEGF vector may be useful in targeting the tumour vasculature in the treatment of breast cancer. © 2001 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23638902009-09-10 Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF Im, S-A Kim, J-S Gomez-Manzano, C Fueyo, J Liu, T-J Cho, M-S Seong, C-M Lee, S N Hong, Y-K Yung, W K A Br J Cancer Regular Article Increased expression of VEGF in several types of tumours has been shown to correlate with poor prognosis. We used a replication-deficient adenoviral vector containing antisense VEGF cDNA (Ad5CMV-αVEGF) to down-regulate VEGF expression and increase the efficiency of delivery of the antisense sequence in the human breast cancer cell line MDA231-MB. Transfection of these cells with Ad5CMV-αVEGF in vitro reduced secreted levels of VEGF protein without affecting cell growth. Moreover, injection of the Ad5CMV-αVEGF vector into intramammary xenografts of these cells established in nude mice inhibited tumour growth and reduced the amount of VEGF protein and the density of microvessels in those tumours relative to tumours treated with the control vector Ad5(dl312). Our results showed that antisense VEGF (165) cDNA was efficiently delivered in vivo via an adenoviral vector and that this treatment significantly inhibited the growth of established experimental breast tumours. The Ad5CMV-αVEGF vector may be useful in targeting the tumour vasculature in the treatment of breast cancer. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-05 /pmc/articles/PMC2363890/ /pubmed/11336478 http://dx.doi.org/10.1054/bjoc.2000.1734 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Im, S-A
Kim, J-S
Gomez-Manzano, C
Fueyo, J
Liu, T-J
Cho, M-S
Seong, C-M
Lee, S N
Hong, Y-K
Yung, W K A
Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title_full Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title_fullStr Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title_full_unstemmed Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title_short Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
title_sort inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-vegf
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363890/
https://www.ncbi.nlm.nih.gov/pubmed/11336478
http://dx.doi.org/10.1054/bjoc.2000.1734
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