Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy

Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood...

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Autores principales: Takano, T, Miyauchi, A, Yoshida, H, Hasegawa, Y, Kuma, K, Amino, N
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363919/
https://www.ncbi.nlm.nih.gov/pubmed/11437410
http://dx.doi.org/10.1054/bjoc.2001.1904
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author Takano, T
Miyauchi, A
Yoshida, H
Hasegawa, Y
Kuma, K
Amino, N
author_facet Takano, T
Miyauchi, A
Yoshida, H
Hasegawa, Y
Kuma, K
Amino, N
author_sort Takano, T
collection PubMed
description Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. © 2001 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23639192009-09-10 Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy Takano, T Miyauchi, A Yoshida, H Hasegawa, Y Kuma, K Amino, N Br J Cancer Regular Article Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-07 /pmc/articles/PMC2363919/ /pubmed/11437410 http://dx.doi.org/10.1054/bjoc.2001.1904 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Takano, T
Miyauchi, A
Yoshida, H
Hasegawa, Y
Kuma, K
Amino, N
Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title_full Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title_fullStr Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title_full_unstemmed Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title_short Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy
title_sort quantitative measurement of thyroglobulin mrna in peripheral blood of patients after total thyroidectomy
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363919/
https://www.ncbi.nlm.nih.gov/pubmed/11437410
http://dx.doi.org/10.1054/bjoc.2001.1904
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