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Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients

Mutations in the K-ras gene are very common in lung tumours and are implicated in the development of lung cancer, but the timing of their occurrence remains poorly understood. We investigated K-ras mutations in cell samples microdissected by laser capture microscopy at multiple sites from lung tissu...

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Autores principales: Keohavong, P, Mady, H H, Gao, W M, Siegfried, J M, Luketich, J D, Melhem, M F
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364035/
https://www.ncbi.nlm.nih.gov/pubmed/11461083
http://dx.doi.org/10.1054/bjoc.2001.1913
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author Keohavong, P
Mady, H H
Gao, W M
Siegfried, J M
Luketich, J D
Melhem, M F
author_facet Keohavong, P
Mady, H H
Gao, W M
Siegfried, J M
Luketich, J D
Melhem, M F
author_sort Keohavong, P
collection PubMed
description Mutations in the K-ras gene are very common in lung tumours and are implicated in the development of lung cancer, but the timing of their occurrence remains poorly understood. We investigated K-ras mutations in cell samples microdissected by laser capture microscopy at multiple sites from lung tissue sections representing tumour tissue and matched histologically normal tissue obtained from 48 lung cancer patients. K-ras mutations were detected in cell samples from 10 of 38 (26.3%) lung adenocarcinomas and in none of the histologically normal or tumour cell samples taken from 10 lung squamous cell carcinomas. Of the K-ras mutation-positive adenocarcinomas, in 4 cases a mutation was found in only the tumour tissue, in 1 case a mutation was found only in the histologically normal tissue, and in 5 cases mutations were found in both the tumour tissue and histologically normal tissue. Among these 5 cases, 2 had identical mutations in both the tumour tissue and histologically normal tissue, 2 had 1 mutation in the tumour tissue and 2 mutations in the histologically normal tissue, 1 of which was identical to the mutation found in the tumour, and 1 case had 2 codon 12 mutations in tumour tissue and 2 mutations, in codons 9 and 11, in histologically normal tissue. These results showed that K-ras mutations are frequent in histologically normal cells taken from outside lung adenocarcinomas and suggest that some of these mutations may represent early events which could pave the way of lung carcinogenesis. © 2001 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23640352009-09-10 Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients Keohavong, P Mady, H H Gao, W M Siegfried, J M Luketich, J D Melhem, M F Br J Cancer Regular Article Mutations in the K-ras gene are very common in lung tumours and are implicated in the development of lung cancer, but the timing of their occurrence remains poorly understood. We investigated K-ras mutations in cell samples microdissected by laser capture microscopy at multiple sites from lung tissue sections representing tumour tissue and matched histologically normal tissue obtained from 48 lung cancer patients. K-ras mutations were detected in cell samples from 10 of 38 (26.3%) lung adenocarcinomas and in none of the histologically normal or tumour cell samples taken from 10 lung squamous cell carcinomas. Of the K-ras mutation-positive adenocarcinomas, in 4 cases a mutation was found in only the tumour tissue, in 1 case a mutation was found only in the histologically normal tissue, and in 5 cases mutations were found in both the tumour tissue and histologically normal tissue. Among these 5 cases, 2 had identical mutations in both the tumour tissue and histologically normal tissue, 2 had 1 mutation in the tumour tissue and 2 mutations in the histologically normal tissue, 1 of which was identical to the mutation found in the tumour, and 1 case had 2 codon 12 mutations in tumour tissue and 2 mutations, in codons 9 and 11, in histologically normal tissue. These results showed that K-ras mutations are frequent in histologically normal cells taken from outside lung adenocarcinomas and suggest that some of these mutations may represent early events which could pave the way of lung carcinogenesis. © 2001 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2001-07 /pmc/articles/PMC2364035/ /pubmed/11461083 http://dx.doi.org/10.1054/bjoc.2001.1913 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Keohavong, P
Mady, H H
Gao, W M
Siegfried, J M
Luketich, J D
Melhem, M F
Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title_full Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title_fullStr Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title_full_unstemmed Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title_short Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
title_sort topographic analysis of k-ras mutations in histologically normal lung tissues and tumours of lung cancer patients
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364035/
https://www.ncbi.nlm.nih.gov/pubmed/11461083
http://dx.doi.org/10.1054/bjoc.2001.1913
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