Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A

NAMI-A is a ruthenium-based compound with selective anti-metastasis activity in experimental models of solid tumours. We studied whether this activity was dependent on anti-angiogenic ability of NAMI-A. We thus investigated its in vitro effects on endothelial cell functions necessary for angiogenesi...

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Autores principales: Vacca, A, Bruno, M, Boccarelli, A, Coluccia, M, Ribatti, D, Bergamo, A, Garbisa, S, Sartor, L, Sava, G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2002
Materias:
Experimental Therapeutics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364145/
https://www.ncbi.nlm.nih.gov/pubmed/11953835
http://dx.doi.org/10.1038/sj.bjc.6600176
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author Vacca, A
Bruno, M
Boccarelli, A
Coluccia, M
Ribatti, D
Bergamo, A
Garbisa, S
Sartor, L
Sava, G
author_facet Vacca, A
Bruno, M
Boccarelli, A
Coluccia, M
Ribatti, D
Bergamo, A
Garbisa, S
Sartor, L
Sava, G
author_sort Vacca, A
collection PubMed
description NAMI-A is a ruthenium-based compound with selective anti-metastasis activity in experimental models of solid tumours. We studied whether this activity was dependent on anti-angiogenic ability of NAMI-A. We thus investigated its in vitro effects on endothelial cell functions necessary for angiogenesis to develop, as well as its in vivo effects in the chick embryo chorioallantoic membrane model. Endothelial cell proliferation, chemotaxis, and secretion of the matrix-degrading enzyme metalloproteinase-2 were inhibited by NAMI-A in a dose-dependent manner, and without morphologic signs of cell apoptosis or necrosis. Lastly, NAMI-A displayed a dose-dependent in vivo anti-angiogenic activity in the chorioallantoic membrane model. These data suggest that the anti-angiogenic activity of NAMI-A can contribute to its anti-metastatic efficacy in mice bearing malignant solid tumours. British Journal of Cancer (2002) 86, 993–998. DOI: 10.1038/sj/bjc/6600176 www.bjcancer.com © 2002 Cancer Research UK
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spelling pubmed-23641452009-09-10 Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A Vacca, A Bruno, M Boccarelli, A Coluccia, M Ribatti, D Bergamo, A Garbisa, S Sartor, L Sava, G Br J Cancer Experimental Therapeutics NAMI-A is a ruthenium-based compound with selective anti-metastasis activity in experimental models of solid tumours. We studied whether this activity was dependent on anti-angiogenic ability of NAMI-A. We thus investigated its in vitro effects on endothelial cell functions necessary for angiogenesis to develop, as well as its in vivo effects in the chick embryo chorioallantoic membrane model. Endothelial cell proliferation, chemotaxis, and secretion of the matrix-degrading enzyme metalloproteinase-2 were inhibited by NAMI-A in a dose-dependent manner, and without morphologic signs of cell apoptosis or necrosis. Lastly, NAMI-A displayed a dose-dependent in vivo anti-angiogenic activity in the chorioallantoic membrane model. These data suggest that the anti-angiogenic activity of NAMI-A can contribute to its anti-metastatic efficacy in mice bearing malignant solid tumours. British Journal of Cancer (2002) 86, 993–998. DOI: 10.1038/sj/bjc/6600176 www.bjcancer.com © 2002 Cancer Research UK Nature Publishing Group 2002-03-18 /pmc/articles/PMC2364145/ /pubmed/11953835 http://dx.doi.org/10.1038/sj.bjc.6600176 Text en Copyright © 2002 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Experimental Therapeutics
Vacca, A
Bruno, M
Boccarelli, A
Coluccia, M
Ribatti, D
Bergamo, A
Garbisa, S
Sartor, L
Sava, G
Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title_full Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title_fullStr Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title_full_unstemmed Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title_short Inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor NAMI-A
title_sort inhibition of endothelial cell functions and of angiogenesis by the metastasis inhibitor nami-a
topic Experimental Therapeutics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364145/
https://www.ncbi.nlm.nih.gov/pubmed/11953835
http://dx.doi.org/10.1038/sj.bjc.6600176
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