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Increased expression of survivin in gastric cancer patients and in first degree relatives
Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric can...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364284/ https://www.ncbi.nlm.nih.gov/pubmed/12085263 http://dx.doi.org/10.1038/sj.bjc.6600421 |
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author | Yu, J Leung, W K Ebert, M P A Ng, E K W Go, M Y Y Wang, H B Chung, S C S Malfertheiner, P Sung, J J Y |
author_facet | Yu, J Leung, W K Ebert, M P A Ng, E K W Go, M Y Y Wang, H B Chung, S C S Malfertheiner, P Sung, J J Y |
author_sort | Yu, J |
collection | PubMed |
description | Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. British Journal of Cancer (2002) 87, 91–97. doi:10.1038/sj.bjc.6600421 www.bjcancer.com © 2002 Cancer Research UK |
format | Text |
id | pubmed-2364284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23642842009-09-10 Increased expression of survivin in gastric cancer patients and in first degree relatives Yu, J Leung, W K Ebert, M P A Ng, E K W Go, M Y Y Wang, H B Chung, S C S Malfertheiner, P Sung, J J Y Br J Cancer Molecular and Cellular Pathology Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. British Journal of Cancer (2002) 87, 91–97. doi:10.1038/sj.bjc.6600421 www.bjcancer.com © 2002 Cancer Research UK Nature Publishing Group 2002-07-01 2002-07-15 /pmc/articles/PMC2364284/ /pubmed/12085263 http://dx.doi.org/10.1038/sj.bjc.6600421 Text en Copyright © 2002 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular and Cellular Pathology Yu, J Leung, W K Ebert, M P A Ng, E K W Go, M Y Y Wang, H B Chung, S C S Malfertheiner, P Sung, J J Y Increased expression of survivin in gastric cancer patients and in first degree relatives |
title | Increased expression of survivin in gastric cancer patients and in first degree relatives |
title_full | Increased expression of survivin in gastric cancer patients and in first degree relatives |
title_fullStr | Increased expression of survivin in gastric cancer patients and in first degree relatives |
title_full_unstemmed | Increased expression of survivin in gastric cancer patients and in first degree relatives |
title_short | Increased expression of survivin in gastric cancer patients and in first degree relatives |
title_sort | increased expression of survivin in gastric cancer patients and in first degree relatives |
topic | Molecular and Cellular Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364284/ https://www.ncbi.nlm.nih.gov/pubmed/12085263 http://dx.doi.org/10.1038/sj.bjc.6600421 |
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