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In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation
Objective: This study was planned to clarify the in vitro effect of lidocaine and bupivacaine on germ tube formation by Candida albicans isolates from cases of clinical vaginal candidiasis. Methods: Fourteen C. albicans strains (clinical vaginal isolates) were grown on Sabouraud agar for 24 h at 37℃...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364336/ https://www.ncbi.nlm.nih.gov/pubmed/18475344 http://dx.doi.org/10.1155/S1064744994000074 |
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author | Rodrigues, Acácio Vaz, Cidália Pina Fonseca, A. Freitas de Oliveira, J. Martinez Barros, Henrique |
author_facet | Rodrigues, Acácio Vaz, Cidália Pina Fonseca, A. Freitas de Oliveira, J. Martinez Barros, Henrique |
author_sort | Rodrigues, Acácio |
collection | PubMed |
description | Objective: This study was planned to clarify the in vitro effect of lidocaine and bupivacaine on germ tube formation by Candida albicans isolates from cases of clinical vaginal candidiasis. Methods: Fourteen C. albicans strains (clinical vaginal isolates) were grown on Sabouraud agar for 24 h at 37℃ and tested as follows: 100 μl of a yeast suspension [10(5) colony forming units (CFU)/ml of phosphate buffered saline (PBS)] was added to 500 μl of fresh human serum with lidocaine or bupivacaine (pure salts) in serial concentrations. The test was run in duplicate. Controls were prepared for each strain. After 4 h of incubation at 37℃, samples were taken from each vial and 200 yeasts were counted in a counting chamber. The pH of each suspension was measured. Results: The results are given as the mean of the 2 readings and are expressed as the percentage of blastoconidia with germ tubes/total blastoconidia. Conclusions: Our experiments show that both lidocaine and bupivacaine have a dose-dependent inhibitory effect, pH-independent, on germ tube formation by C. albicans and that both drugs seem to be promising in the treatment of genital candidiasis due to the combination of anesthetic and antifungal properties. |
format | Text |
id | pubmed-2364336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23643362008-05-12 In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation Rodrigues, Acácio Vaz, Cidália Pina Fonseca, A. Freitas de Oliveira, J. Martinez Barros, Henrique Infect Dis Obstet Gynecol Research Article Objective: This study was planned to clarify the in vitro effect of lidocaine and bupivacaine on germ tube formation by Candida albicans isolates from cases of clinical vaginal candidiasis. Methods: Fourteen C. albicans strains (clinical vaginal isolates) were grown on Sabouraud agar for 24 h at 37℃ and tested as follows: 100 μl of a yeast suspension [10(5) colony forming units (CFU)/ml of phosphate buffered saline (PBS)] was added to 500 μl of fresh human serum with lidocaine or bupivacaine (pure salts) in serial concentrations. The test was run in duplicate. Controls were prepared for each strain. After 4 h of incubation at 37℃, samples were taken from each vial and 200 yeasts were counted in a counting chamber. The pH of each suspension was measured. Results: The results are given as the mean of the 2 readings and are expressed as the percentage of blastoconidia with germ tubes/total blastoconidia. Conclusions: Our experiments show that both lidocaine and bupivacaine have a dose-dependent inhibitory effect, pH-independent, on germ tube formation by C. albicans and that both drugs seem to be promising in the treatment of genital candidiasis due to the combination of anesthetic and antifungal properties. Hindawi Publishing Corporation 1994 /pmc/articles/PMC2364336/ /pubmed/18475344 http://dx.doi.org/10.1155/S1064744994000074 Text en Copyright © 1994 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rodrigues, Acácio Vaz, Cidália Pina Fonseca, A. Freitas de Oliveira, J. Martinez Barros, Henrique In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title | In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title_full | In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title_fullStr | In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title_full_unstemmed | In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title_short | In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation |
title_sort | in vitro effect of local anesthetics on candida albicans germ tube formation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364336/ https://www.ncbi.nlm.nih.gov/pubmed/18475344 http://dx.doi.org/10.1155/S1064744994000074 |
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