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Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting
Objective: Streptococcus pyogenes is the cause of the classical childbed fever and can occur in both sporadic and epidemic form. Once an outbreak is identified on a maternity ward it is not only necessary to place the patients in strict isolation but also identify to the source of the infection. Fas...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364544/ https://www.ncbi.nlm.nih.gov/pubmed/18476143 http://dx.doi.org/10.1155/S1064744997000392 |
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author | Meis, Jacques F. G. M. Muytjens, Harry L. van den Berg, Paul P. Voss, Andreas Melchers, Willem J. G. |
author_facet | Meis, Jacques F. G. M. Muytjens, Harry L. van den Berg, Paul P. Voss, Andreas Melchers, Willem J. G. |
author_sort | Meis, Jacques F. G. M. |
collection | PubMed |
description | Objective: Streptococcus pyogenes is the cause of the classical childbed fever and can occur in both sporadic and epidemic form. Once an outbreak is identified on a maternity ward it is not only necessary to place the patients in strict isolation but also identify to the source of the infection. Fast reliable typing methods can aid in infection control. Methods: An outbreak of puerperal fever due to S. pyogenes was analyzed by random amplified polymorphic DNA (RAPD) analysis. Results: Identical fingerprint patterns were found in isolates of 3 patients, the throat and infected finger of the delivering obstetrician, 2 of the physician's family members, and from the cervix of a woman who was examined by the physician 7 months after the outbreak. The outbreak was stopped after antimicrobial treatment of the physician and his family members. Conclusions: RAPD typing appeared to be a fast and reliable tool for epidemiological studies of S. pyogenes and is probably more efficient in strain differentiation than classical M and T serotyping. |
format | Text |
id | pubmed-2364544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23645442008-05-12 Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting Meis, Jacques F. G. M. Muytjens, Harry L. van den Berg, Paul P. Voss, Andreas Melchers, Willem J. G. Infect Dis Obstet Gynecol Research Article Objective: Streptococcus pyogenes is the cause of the classical childbed fever and can occur in both sporadic and epidemic form. Once an outbreak is identified on a maternity ward it is not only necessary to place the patients in strict isolation but also identify to the source of the infection. Fast reliable typing methods can aid in infection control. Methods: An outbreak of puerperal fever due to S. pyogenes was analyzed by random amplified polymorphic DNA (RAPD) analysis. Results: Identical fingerprint patterns were found in isolates of 3 patients, the throat and infected finger of the delivering obstetrician, 2 of the physician's family members, and from the cervix of a woman who was examined by the physician 7 months after the outbreak. The outbreak was stopped after antimicrobial treatment of the physician and his family members. Conclusions: RAPD typing appeared to be a fast and reliable tool for epidemiological studies of S. pyogenes and is probably more efficient in strain differentiation than classical M and T serotyping. Hindawi Publishing Corporation 1997 /pmc/articles/PMC2364544/ /pubmed/18476143 http://dx.doi.org/10.1155/S1064744997000392 Text en Copyright © 1997 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Meis, Jacques F. G. M. Muytjens, Harry L. van den Berg, Paul P. Voss, Andreas Melchers, Willem J. G. Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title | Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title_full | Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title_fullStr | Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title_full_unstemmed | Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title_short | Analysis of an Outbreak of Puerperal Fever Due to Group A Streptococci by Random Amplified Polymorphic DNA Fingerprinting |
title_sort | analysis of an outbreak of puerperal fever due to group a streptococci by random amplified polymorphic dna fingerprinting |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364544/ https://www.ncbi.nlm.nih.gov/pubmed/18476143 http://dx.doi.org/10.1155/S1064744997000392 |
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