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Transport of the Dicyanogold(I) Anion
We have shown that dicyanogold(I), [Au(CN)(2)](-) is a common metabolite found in blood and urine samples of patients treated with different gold based drugs. Some patients have high levels of gold within their red blood cells (RBCs). Size exclusion and C18 reversed phase chromatography show that th...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364916/ https://www.ncbi.nlm.nih.gov/pubmed/18476261 http://dx.doi.org/10.1155/MBD.1994.433 |
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author | Tepperman, Katherine Zhang, Yafei Roy, Pamela W. Floyd, Roger Zhao, Zheng Dorsey, John G. Elder, R. C. |
author_facet | Tepperman, Katherine Zhang, Yafei Roy, Pamela W. Floyd, Roger Zhao, Zheng Dorsey, John G. Elder, R. C. |
author_sort | Tepperman, Katherine |
collection | PubMed |
description | We have shown that dicyanogold(I), [Au(CN)(2)](-) is a common metabolite found in blood and urine samples of patients treated with different gold based drugs. Some patients have high levels of gold within their red blood cells (RBCs). Size exclusion and C18 reversed phase chromatography show that the majority of the gold in RBC lysates is bound to protein, but small molecules such as dicyanogold(I) and gold-glutathione complexes are also present. Dicyanogold incubation with red blood cells in vitro leads to a rapid and complete uptake of gold. This uptake is unaffected by DIDS, an inhibitor of the anion channel, but is blocked by the addition of external cyanide. Dicyanogold is also readily taken up by H9 cells, a continuous CD(4+) cell line. This uptake is significantly inhibited by N-ethylmaleimide, suggesting a requirement for sulfhydryl groups. Dicyanogold inhibits the replication of the AIDS virus, HIV, in a cell culture model. |
format | Text |
id | pubmed-2364916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23649162008-05-12 Transport of the Dicyanogold(I) Anion Tepperman, Katherine Zhang, Yafei Roy, Pamela W. Floyd, Roger Zhao, Zheng Dorsey, John G. Elder, R. C. Met Based Drugs Research Article We have shown that dicyanogold(I), [Au(CN)(2)](-) is a common metabolite found in blood and urine samples of patients treated with different gold based drugs. Some patients have high levels of gold within their red blood cells (RBCs). Size exclusion and C18 reversed phase chromatography show that the majority of the gold in RBC lysates is bound to protein, but small molecules such as dicyanogold(I) and gold-glutathione complexes are also present. Dicyanogold incubation with red blood cells in vitro leads to a rapid and complete uptake of gold. This uptake is unaffected by DIDS, an inhibitor of the anion channel, but is blocked by the addition of external cyanide. Dicyanogold is also readily taken up by H9 cells, a continuous CD(4+) cell line. This uptake is significantly inhibited by N-ethylmaleimide, suggesting a requirement for sulfhydryl groups. Dicyanogold inhibits the replication of the AIDS virus, HIV, in a cell culture model. Hindawi Publishing Corporation 1994 /pmc/articles/PMC2364916/ /pubmed/18476261 http://dx.doi.org/10.1155/MBD.1994.433 Text en Copyright © 1994 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tepperman, Katherine Zhang, Yafei Roy, Pamela W. Floyd, Roger Zhao, Zheng Dorsey, John G. Elder, R. C. Transport of the Dicyanogold(I) Anion |
title | Transport of the Dicyanogold(I) Anion |
title_full | Transport of the Dicyanogold(I) Anion |
title_fullStr | Transport of the Dicyanogold(I) Anion |
title_full_unstemmed | Transport of the Dicyanogold(I) Anion |
title_short | Transport of the Dicyanogold(I) Anion |
title_sort | transport of the dicyanogold(i) anion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364916/ https://www.ncbi.nlm.nih.gov/pubmed/18476261 http://dx.doi.org/10.1155/MBD.1994.433 |
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