Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers

Oral delivery of proteins and peptides as therapeutic agents is problematic due to their low bioavailability. This study examined the effect of boronation on the transepithelial transport and metabolism of three glycine-phenylalanine dipeptides in Caco-2 and HCT-8 cell monolayers. The three dipeptid...

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Detalles Bibliográficos
Autores principales: Elkins, Amy L., Eley, John G., Miller III, Merrill C., Hall, Iris H., Sood, Anup, Spielvogel, Bernard
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365028/
https://www.ncbi.nlm.nih.gov/pubmed/18475758
http://dx.doi.org/10.1155/MBD.1996.277
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author Elkins, Amy L.
Eley, John G.
Miller III, Merrill C.
Hall, Iris H.
Sood, Anup
Spielvogel, Bernard
author_facet Elkins, Amy L.
Eley, John G.
Miller III, Merrill C.
Hall, Iris H.
Sood, Anup
Spielvogel, Bernard
author_sort Elkins, Amy L.
collection PubMed
description Oral delivery of proteins and peptides as therapeutic agents is problematic due to their low bioavailability. This study examined the effect of boronation on the transepithelial transport and metabolism of three glycine-phenylalanine dipeptides in Caco-2 and HCT-8 cell monolayers. The three dipeptides exhibited passive transport characteristics in the monolayer systems. However, metabolism of the boronated dipeptides did occur, but to a lesser extent than the non-boronated glycine-phenylalanine dipeptide. The same metabolic scheme was seen in both cell monolayer system, but greater metabolism was seen in the HCT-8 cell monolayers.
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spelling pubmed-23650282008-05-12 Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers Elkins, Amy L. Eley, John G. Miller III, Merrill C. Hall, Iris H. Sood, Anup Spielvogel, Bernard Met Based Drugs Research Article Oral delivery of proteins and peptides as therapeutic agents is problematic due to their low bioavailability. This study examined the effect of boronation on the transepithelial transport and metabolism of three glycine-phenylalanine dipeptides in Caco-2 and HCT-8 cell monolayers. The three dipeptides exhibited passive transport characteristics in the monolayer systems. However, metabolism of the boronated dipeptides did occur, but to a lesser extent than the non-boronated glycine-phenylalanine dipeptide. The same metabolic scheme was seen in both cell monolayer system, but greater metabolism was seen in the HCT-8 cell monolayers. Hindawi Publishing Corporation 1996 /pmc/articles/PMC2365028/ /pubmed/18475758 http://dx.doi.org/10.1155/MBD.1996.277 Text en Copyright © 1996 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Elkins, Amy L.
Eley, John G.
Miller III, Merrill C.
Hall, Iris H.
Sood, Anup
Spielvogel, Bernard
Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title_full Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title_fullStr Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title_full_unstemmed Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title_short Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers
title_sort transepithelial transport and metabolism of boronated dipeptides across caco-2 and hct-8 cell monolayers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365028/
https://www.ncbi.nlm.nih.gov/pubmed/18475758
http://dx.doi.org/10.1155/MBD.1996.277
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