Cargando…
Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment
In rats cisplatin induces hypocalcemia, bloating of the stomach, and ulceration ameliorated through calcium supplements. This study was undertaken to test the role of calcium on the gastrin mRNA production in vitro and in vivo. RIN B6 cells were cultured in medium with calcium (1.8, 3.6 and 7.2 mM)...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2000
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365213/ https://www.ncbi.nlm.nih.gov/pubmed/18475933 http://dx.doi.org/10.1155/MBD.2000.115 |
_version_ | 1782154114003632128 |
---|---|
author | Wang, Ying Aggarwal, Surinder K. Kopachik, Will |
author_facet | Wang, Ying Aggarwal, Surinder K. Kopachik, Will |
author_sort | Wang, Ying |
collection | PubMed |
description | In rats cisplatin induces hypocalcemia, bloating of the stomach, and ulceration ameliorated through calcium supplements. This study was undertaken to test the role of calcium on the gastrin mRNA production in vitro and in vivo. RIN B6 cells were cultured in medium with calcium (1.8, 3.6 and 7.2 mM) and the active form of vitamin D (calcijex). Cisplatin was added (10 μg/ml) for 12 hrs and cells were harvested for RNA from various treatment groups. Male Wistar rats were treated with cisplatin (9 mg/kg), before and after vitamin D (0.3 mg/100g/week). The rats were killed and stomach tissues excised on 1, 6, 10 and 15 days after cisplatin treatment. RNA from the stomach was analyzed using the northern blot technique. Gastrin mRNA was suppressed after cisplatin treatment both in vitro and in vivo. In vitro calcium but not vitamin D additions partially prevented the gastrin mRNA. In vivo, however, vitamin D and calcium were equally effective in preventing gastrin mRNA loss. |
format | Text |
id | pubmed-2365213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23652132008-05-12 Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment Wang, Ying Aggarwal, Surinder K. Kopachik, Will Met Based Drugs Research Article In rats cisplatin induces hypocalcemia, bloating of the stomach, and ulceration ameliorated through calcium supplements. This study was undertaken to test the role of calcium on the gastrin mRNA production in vitro and in vivo. RIN B6 cells were cultured in medium with calcium (1.8, 3.6 and 7.2 mM) and the active form of vitamin D (calcijex). Cisplatin was added (10 μg/ml) for 12 hrs and cells were harvested for RNA from various treatment groups. Male Wistar rats were treated with cisplatin (9 mg/kg), before and after vitamin D (0.3 mg/100g/week). The rats were killed and stomach tissues excised on 1, 6, 10 and 15 days after cisplatin treatment. RNA from the stomach was analyzed using the northern blot technique. Gastrin mRNA was suppressed after cisplatin treatment both in vitro and in vivo. In vitro calcium but not vitamin D additions partially prevented the gastrin mRNA. In vivo, however, vitamin D and calcium were equally effective in preventing gastrin mRNA loss. Hindawi Publishing Corporation 2000 /pmc/articles/PMC2365213/ /pubmed/18475933 http://dx.doi.org/10.1155/MBD.2000.115 Text en Copyright © 2000 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Ying Aggarwal, Surinder K. Kopachik, Will Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title | Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title_full | Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title_fullStr | Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title_full_unstemmed | Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title_short | Role of Vitamin D on the Inhibition of Gastrin Production After Cisplatin Treatment |
title_sort | role of vitamin d on the inhibition of gastrin production after cisplatin treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365213/ https://www.ncbi.nlm.nih.gov/pubmed/18475933 http://dx.doi.org/10.1155/MBD.2000.115 |
work_keys_str_mv | AT wangying roleofvitamindontheinhibitionofgastrinproductionaftercisplatintreatment AT aggarwalsurinderk roleofvitamindontheinhibitionofgastrinproductionaftercisplatintreatment AT kopachikwill roleofvitamindontheinhibitionofgastrinproductionaftercisplatintreatment |