IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture

Incubation of rat hepatocytes in primary culture with IL-1β at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (−35%) in the amount of apoE mRNA, but increas...

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Detalles Bibliográficos
Autores principales: Ribeiro, Agnes, Mangeney, Marise, Bernard, Isabelle, Chambaz, Jean, Delers, Francisco
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365358/
https://www.ncbi.nlm.nih.gov/pubmed/18475480
http://dx.doi.org/10.1155/S0962935192000498
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author Ribeiro, Agnes
Mangeney, Marise
Bernard, Isabelle
Chambaz, Jean
Delers, Francisco
author_facet Ribeiro, Agnes
Mangeney, Marise
Bernard, Isabelle
Chambaz, Jean
Delers, Francisco
author_sort Ribeiro, Agnes
collection PubMed
description Incubation of rat hepatocytes in primary culture with IL-1β at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (−35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1β (10 units/ml) or IL-6 (100 units/ml). These results suggest that inflammatory cytokines IL-1β and IL-6 modulate the expression of apoE gene in cultured rat hepatocytes, at a concentration that does not induce the acute phase response.
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spelling pubmed-23653582008-05-12 IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture Ribeiro, Agnes Mangeney, Marise Bernard, Isabelle Chambaz, Jean Delers, Francisco Mediators Inflamm Research Article Incubation of rat hepatocytes in primary culture with IL-1β at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (−35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1β (10 units/ml) or IL-6 (100 units/ml). These results suggest that inflammatory cytokines IL-1β and IL-6 modulate the expression of apoE gene in cultured rat hepatocytes, at a concentration that does not induce the acute phase response. Hindawi Publishing Corporation 1992 /pmc/articles/PMC2365358/ /pubmed/18475480 http://dx.doi.org/10.1155/S0962935192000498 Text en Copyright © 1992 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ribeiro, Agnes
Mangeney, Marise
Bernard, Isabelle
Chambaz, Jean
Delers, Francisco
IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title_full IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title_fullStr IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title_full_unstemmed IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title_short IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
title_sort il-1β and il-6 modulate apolipoprotein e gene expression in rat hepatocyte primary culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365358/
https://www.ncbi.nlm.nih.gov/pubmed/18475480
http://dx.doi.org/10.1155/S0962935192000498
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