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Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils
Melittin, the predominant fraction of bee venom proteins, was studied in an experimental model of human neutrophil granulocytes to reveal its influence on eicosanoid release, metabolism and receptor function in relation to intracellular calcium metabolism. Melittin (2 μmol/l) was as potent as the ca...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365360/ https://www.ncbi.nlm.nih.gov/pubmed/18475477 http://dx.doi.org/10.1155/S0962935192000462 |
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author | Nielsen, Ole H. Bouchelouche, Pierre N. Berild, Dag |
author_facet | Nielsen, Ole H. Bouchelouche, Pierre N. Berild, Dag |
author_sort | Nielsen, Ole H. |
collection | PubMed |
description | Melittin, the predominant fraction of bee venom proteins, was studied in an experimental model of human neutrophil granulocytes to reveal its influence on eicosanoid release, metabolism and receptor function in relation to intracellular calcium metabolism. Melittin (2 μmol/l) was as potent as the calcium ionophore A23187 (10 μmol/l) for activation of 5-lipoxygenase, releasing arachidonate only from phosphatidyl-choline and phosphatidyl-ethanolamine of cellular membranes, as judged from the decreases in radioactivity by 15.4% and 30.5%, respectively. The mechanism responsible for the release of arachidonate from cellular membranes is closely coupled to cellular calcium metabolism, and melittin was found to promote calcium entry through receptor gated calcium channels, probably due to an activation of phospholipase A(2). Furthermore, a down-regulation of leukotriene B(4) receptors was seen. The maximal number of binding sites per cell was reduced from a median of 1520 to 950 with melittin (1 μmol/l). The study has revealed some factors important for the inflammatory mechanisms mediated by melittin. |
format | Text |
id | pubmed-2365360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23653602008-05-12 Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils Nielsen, Ole H. Bouchelouche, Pierre N. Berild, Dag Mediators Inflamm Research Article Melittin, the predominant fraction of bee venom proteins, was studied in an experimental model of human neutrophil granulocytes to reveal its influence on eicosanoid release, metabolism and receptor function in relation to intracellular calcium metabolism. Melittin (2 μmol/l) was as potent as the calcium ionophore A23187 (10 μmol/l) for activation of 5-lipoxygenase, releasing arachidonate only from phosphatidyl-choline and phosphatidyl-ethanolamine of cellular membranes, as judged from the decreases in radioactivity by 15.4% and 30.5%, respectively. The mechanism responsible for the release of arachidonate from cellular membranes is closely coupled to cellular calcium metabolism, and melittin was found to promote calcium entry through receptor gated calcium channels, probably due to an activation of phospholipase A(2). Furthermore, a down-regulation of leukotriene B(4) receptors was seen. The maximal number of binding sites per cell was reduced from a median of 1520 to 950 with melittin (1 μmol/l). The study has revealed some factors important for the inflammatory mechanisms mediated by melittin. Hindawi Publishing Corporation 1992 /pmc/articles/PMC2365360/ /pubmed/18475477 http://dx.doi.org/10.1155/S0962935192000462 Text en Copyright © 1992 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nielsen, Ole H. Bouchelouche, Pierre N. Berild, Dag Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title | Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title_full | Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title_fullStr | Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title_full_unstemmed | Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title_short | Arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
title_sort | arachidonic acid and calcium metabolism in rnelittin stimulated neutrophils |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365360/ https://www.ncbi.nlm.nih.gov/pubmed/18475477 http://dx.doi.org/10.1155/S0962935192000462 |
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